Zhao Xin, Cheng Qiang, Qian Yu, Yi Ruokun, Gu Lianjie, Wang Shanshan, Song Jia-Le
Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, P.R. China.
Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, P.R. China.
Exp Ther Med. 2017 Nov;14(5):5135-5142. doi: 10.3892/etm.2017.5181. Epub 2017 Sep 22.
The aim of the present study was to investigate the protective effect of insect tea on HCl/ethanol-induced gastric ulcers in ICR mice. The serum levels of vasoactive intestinal peptide, substance P, somatostatin, motilin and endothelin-1 in mice were assessed using commercial kits and gastric tissues of superoxide dismutase (SOD, nitric oxide (NO) and malondialdehyde (MDA) were determined using western blot analysis Insect tea significantly reduced HCl/ethanol-induced gastric juice secretion and increased the pH of gastric juice (P<0.05). Insect tea treatment signfiicantly increased vasoactive intestinal peptide and somatostatin, and significantly decreased motilin, substance P and endothelin levels in the serum (P<0.05). Treatment with insect tea was demonstrated to significantly increase levels of gastric SOD and NO and to reduce levels of MDA in the gastric ulcer mouse model (P<0.05). The gastric expression of inhibitor of nuclear factor-κB (NF-κB), epidermal growth factor (EGF), EGF receptor, neuronal nitric oxide synthase (nNOS), endothelial NOS, Mn-SOD, Cu/Zn-SOD and catalase was significantly increased in mice treated with inset tea compared with untreated model mice (P<0.05). Levels of NF-κB, and inducible NOS were demonstrated to be decreased in mice treated with insect tea compared with untreated model mice (P<0.05). The results of the present study suggest that insect tea has a protective effect against HCl/ethanol-induced gastric ulcers in ICR mice. This effect may be achieved via modulating serum neuropeptide levels, reducing gastric juice secretion, and modulating the inflammation- and antioxidant-associated protein expressions in gastric tissue.
本研究的目的是探讨虫茶对盐酸/乙醇诱导的ICR小鼠胃溃疡的保护作用。使用商业试剂盒评估小鼠血清中血管活性肠肽、P物质、生长抑素、胃动素和内皮素-1的水平，并通过蛋白质印迹分析测定胃组织中超氧化物歧化酶（SOD）、一氧化氮（NO）和丙二醛（MDA）的含量。虫茶显著减少了盐酸/乙醇诱导的胃液分泌，并提高了胃液的pH值（P<0.05）。虫茶处理显著增加了血清中血管活性肠肽和生长抑素的水平，并显著降低了胃动素、P物质和内皮素的水平（P<0.05）。在胃溃疡小鼠模型中，虫茶治疗可显著提高胃SOD和NO的水平，并降低MDA的水平（P<0.05）。与未处理的模型小鼠相比，用虫茶处理的小鼠胃中核因子-κB（NF-κB）抑制剂、表皮生长因子（EGF）、EGF受体、神经元型一氧化氮合酶（nNOS）、内皮型一氧化氮合酶、锰超氧化物歧化酶、铜/锌超氧化物歧化酶和过氧化氢酶的表达显著增加（P<0.05）。与未处理的模型小鼠相比，用虫茶处理的小鼠中NF-κB和诱导型一氧化氮合酶的水平降低（P<0.05）。本研究结果表明，虫茶对盐酸/乙醇诱导的ICR小鼠胃溃疡具有保护作用。这种作用可能是通过调节血清神经肽水平、减少胃液分泌以及调节胃组织中与炎症和抗氧化相关的蛋白表达来实现的。
