Shahbazfar Amir Ali, Zare Payman, Ranjbaran Mehrdad, Tayefi-Nasrabadi Hossein, Fakhri Omid, Farshi Yashar, Shadi Sahar, Khoshkerdar Afsaneh
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
J Cancer Res Ther. 2014 Oct-Dec;10(4):1057-62. doi: 10.4103/0973-1482.137975.
Anticancer properties of artemisinin and its derivatives have been shown in many experiments.
Addition of butyric acid, miconazole, and iron to this traditional drug has been done in order to enhance its anticancer potency.
Cell lines 5637 and 4T1, were cultivated and classified into 13 groups of three each. Different doses of artemisinin with constant doses of iron, miconazole and butyric acid, were added to the cultures. At the end of exposure pathological and enzymatic studies were performed.
In four groups treated with different doses of artemisinin and iron, dose-dependent changes were observed. These changes included apoptosis and necrosis with dominance of apoptosis. The supernatant lactate dehydrogenase (LDH) level was increased in a dose-dependent manner, but there was no significant increase in the cell fraction of malonyldialdehyde (MDA) or LDH. In four other groups, which received miconazole, butyric acid and iron in addition to different doses of artemisinin, necrosis was more prominent than apoptosis, and the MDA level did not show any significant change, but LDH was increased. The groups treated with miconazole showed identical changes, with less severity compared to combination therapy groups. In butyric acid-treated groups, the only detectable changes were, mild cell swelling, few apoptosis, and rare necrosis.
A combination therapy with artemisinin can be more effective against cancer cells than monotherapy with that. Butyric acid was not effective on cancer cells. Miconazole deviated the nature of cell death from apoptosis to necrosis and it must be used under caution.
青蒿素及其衍生物的抗癌特性已在许多实验中得到证实。
为增强这种传统药物的抗癌效力，已将丁酸、咪康唑和铁添加到其中。
培养5637和4T1细胞系，并将其分为13组，每组三个。将不同剂量的青蒿素与恒定剂量的铁、咪康唑和丁酸添加到培养物中。在暴露结束时进行病理和酶学研究。
在用不同剂量的青蒿素和铁处理的四组中，观察到剂量依赖性变化。这些变化包括凋亡和坏死，凋亡占主导。上清液乳酸脱氢酶（LDH）水平呈剂量依赖性升高，但丙二醛（MDA）或LDH的细胞部分无显著增加。在另外四组中，除了不同剂量的青蒿素外，还接受了咪康唑、丁酸和铁，坏死比凋亡更明显，MDA水平无显著变化，但LDH升高。用咪康唑处理的组表现出相同的变化，与联合治疗组相比严重程度较低。在丁酸处理的组中，唯一可检测到的变化是轻度细胞肿胀、少量凋亡和罕见坏死。
青蒿素联合治疗对癌细胞可能比单一疗法更有效。丁酸对癌细胞无效。咪康唑使细胞死亡的性质从凋亡转变为坏死，必须谨慎使用。
