Wang Hai-Yan, Chen Yun-Yun, Zhang Yu-Qing
Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, 702-2303 Room, No. 199, Renai Road, Dushuhu Higher Edu. Town, Suzhou 215123, PR China.
Silk Biotechnology Laboratory, School of Biology and Basic Medical Sciences, Soochow University, 702-2303 Room, No. 199, Renai Road, Dushuhu Higher Edu. Town, Suzhou 215123, PR China.
Mater Sci Eng C Mater Biol Appl. 2015 Mar;48:444-52. doi: 10.1016/j.msec.2014.12.028. Epub 2014 Dec 11.
Silk derived from Bombyx mori silkworm cocoons was degummed in an aqueous sodium carbonate solution, and the resulting silk fibroin fibers were placed in an acidic aqueous solution and were treated with ultrasonication to obtain powdered micro- and nanofibers. The morphologies and spectral characteristics of these powdered silk fibers were investigated in detail. The shape, surface and structural features of the powdered fibers were affected by the ultrasonic power and media. Increasing the acidity of the ultrasonic solution and increasing the ultrasonic power increased the fiber breakage speed, resulting in shorter fiber lengths. Powdered microfibers could not be obtained in a formic acid solution, while powdered nanofibers whose diameter below 1μm were obtained in a combined formic acid and hydrochloric acid ultrasonication solution. Observation via SEM and optical microscopy revealed that the microfiber diameters were approximately 5-10μm, and those of the nanofibers were approximately 30-120nm. The analysis of laser sizer showed that the microfiber sizes ranged mainly from 20 to 100μm. FT-IR and XRD spectra demonstrated that the relative amount of β-sheets increased after the ultrasonic treatment. The ε-amino group content on the surface of the micro- and nanofibers increased significantly. These studies provide reliable methods for the preparation of nano-scale silk fibroin fibers by ultrasonication and open new avenues for the development of powdered silk fibers as advanced functional biomaterials.
将家蚕茧缫得的丝在碳酸钠水溶液中脱胶,然后将所得的丝素纤维置于酸性水溶液中,并用超声处理以获得粉末状的微纤维和纳米纤维。详细研究了这些粉末状丝纤维的形态和光谱特征。粉末状纤维的形状、表面和结构特征受超声功率和介质的影响。提高超声溶液的酸度和增加超声功率会提高纤维的断裂速度,导致纤维长度变短。在甲酸溶液中无法获得粉末状微纤维,而在甲酸和盐酸混合超声溶液中获得了直径低于1μm的粉末状纳米纤维。通过扫描电子显微镜(SEM)和光学显微镜观察发现,微纤维直径约为5-10μm,纳米纤维直径约为30-120nm。激光粒度分析仪分析表明,微纤维尺寸主要在20至100μm范围内。傅里叶变换红外光谱(FT-IR)和X射线衍射(XRD)光谱表明,超声处理后β-折叠的相对含量增加。微纤维和纳米纤维表面的ε-氨基含量显著增加。这些研究为通过超声处理制备纳米级丝素纤维提供了可靠的方法,并为开发作为先进功能生物材料的粉末状丝纤维开辟了新途径。