特定黄酮醇在活化的RAW 264.7巨噬细胞上肿瘤坏死因子相关凋亡诱导配体受体-1（TRAIL-R1）表达中的作用
The role of selected flavonols in tumor necrosis factor-related apoptosis-inducing ligand receptor-1 (TRAIL-R1) expression on activated RAW 264.7 macrophages.
作者信息
Warat Monika, Sadowski Tadeusz, Szliszka Ewelina, Król Wojciech, Czuba Zenon P
机构信息
School of Medicine with the Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Chair and Department of Microbiology and Immunology, Jordana 19, 41-808 Zabrze, Poland.
School of Public Health in Bytom, Medical University of Silesia in Katowice, Toxicology and Drug Addiction Division, Communal Department of Hygiene and Sanitary Supervision, Medyków 18, 40-752 Katowice, Poland.
出版信息
Molecules. 2015 Jan 8;20(1):900-12. doi: 10.3390/molecules20010900.
Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptors (TRAIL-R) are an important factor of apoptosis in cancer cells. There are no data about the effect of flavonols on the receptor expression on a surface of macrophage like cells. In this study, the expression level of TRAIL-R1 on murine RAW264.7 macrophages in the presence of selected flavonols: galangin, kaempferol, kaempferide and quercetin, which differ from their phenyl ring substituents, were studied. The expression of TRAIL-R1 death receptors on non-stimulated and lipopolysaccharide (LPS)-stimulated macrophages was determined using flow cytometry. The results suggested that compounds being tested can modulate TRAIL-R1 expression and can enhance TRAIL-mediated apoptosis.
肿瘤坏死因子相关凋亡诱导配体受体（TRAIL-R）是癌细胞凋亡的一个重要因素。目前尚无关于黄酮醇对巨噬细胞样细胞表面受体表达影响的数据。在本研究中，研究了在存在选定黄酮醇（高良姜素、山奈酚、山奈素和槲皮素，它们的苯环取代基不同）的情况下，小鼠RAW264.7巨噬细胞上TRAIL-R1的表达水平。使用流式细胞术测定未刺激和脂多糖（LPS）刺激的巨噬细胞上TRAIL-R1死亡受体的表达。结果表明，所测试的化合物可以调节TRAIL-R1的表达，并可以增强TRAIL介导的凋亡。
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