使用寡核苷酸探针（CAC）5/（GTG）5进行DNA指纹分析：体细胞稳定性和种系突变
DNA fingerprinting with the oligonucleotide probe (CAC)5/(GTG)5: somatic stability and germline mutations.
作者信息
Nürnberg P, Roewer L, Neitzel H, Sperling K, Pöpperl A, Hundrieser J, Pöche H, Epplen C, Zischler H, Epplen J T
机构信息
Institut für Medizinische Genetik des Bereichs Medizin der Charité, Humboldt-Universität, Berlin, German Democratic Republic.
出版信息
Hum Genet. 1989 Dec;84(1):75-8. doi: 10.1007/BF00210676.
DNA fingerprints were generated from various human somatic tissues and from peripheral blood of 179 children and their 80 parents using (CAC)5/(GTG)5 oligonucleotide probes. Whereas somatic stability of the fingerprint patterns was demonstrated, the average rate for germline mutations was estimated to be approximately 0.001 per DNA locus and gamete, with the three different restriction enzymes used. Seven out of eight mutations observed appeared to be of paternal origin.
使用（CAC）5/（GTG）5寡核苷酸探针，从179名儿童及其80名父母的各种人体体细胞组织和外周血中生成了DNA指纹。虽然证明了指纹图谱的体细胞稳定性，但使用三种不同的限制性内切酶时，种系突变的平均发生率估计约为每个DNA位点和配子0.001。观察到的8个突变中有7个似乎来自父系。
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