Egorov A A, Ambartsumova N V
Mikrobiol Zh (1978). 1989 Jul-Aug;51(4):95-7.
Optimal conditions to reveal the hemagglutinating activity in the Coxsackie viruses of group B and hemagglutination test (HAT) performed by the micromethod are studied and determined. The titre of hemagglutinins revealed in HAT is established to depend on pH of the phosphate-buffer solution, concentration of human erythrocytes and their group attribution. The viruses passaged at the suboptimal temperature 33 degrees C are shown to possess the maximal hemagglutinating activity.
研究并确定了揭示B组柯萨奇病毒血凝活性的最佳条件以及采用微量法进行的血凝试验(HAT)。结果表明,HAT中所揭示的血凝素滴度取决于磷酸盐缓冲溶液的pH值、人红细胞的浓度及其血型归属。在33摄氏度次优温度下传代的病毒显示出最大的血凝活性。