Kende M, Uj M, Szücs G
Acta Microbiol Acad Sci Hung. 1979;26(3):179-84.
Among the widely applied buffered media, the HSAG (hepes-salt-albumin-gelatin) medium at pH 5.75--6.25 was found to be the most favourable for B.K. virus haemagglutinin titration. The optimum temperature was at 4 degrees C. The haemagglutinin was not affected by temperatures up to 37 degrees C, pHs between 5.5 and 9.5, and NaCl concentrations between 0.063 M and 2.56 M. When incubated at 56 degrees C, the haemagglutinin shows a time and pH dependent decline in titre. No significant time dependent titre fall occurred at 56 degrees C if NaCl molarity was varied between 1.31 and 2.56.
在广泛应用的缓冲介质中，发现pH值为5.75至6.25的HSAG（羟乙基哌嗪乙磺酸-盐-白蛋白-明胶）培养基最有利于BK病毒血凝素滴定。最适温度为4℃。血凝素不受高达37℃的温度、5.5至9.5的pH值以及0.063 M至2.56 M的氯化钠浓度的影响。当在56℃孵育时，血凝素的滴度呈现出时间和pH依赖性下降。如果氯化钠摩尔浓度在1.31至2.56之间变化，在56℃时不会出现显著的时间依赖性滴度下降。
