Dahmani Mustapha, Loudahi Abdelghani, Mediannikov Oleg, Fenollar Florence, Raoult Didier, Davoust Bernard
Research Unit of Emerging Infectious and Tropical Diseases (URMITE) UMR CNRS, 7278 IRD 198 INSERM 1095, Aix-Marseille University, Marseille, France.
Research Unit of Emerging Infectious and Tropical Diseases (URMITE) UMR CNRS, 7278 IRD 198 INSERM 1095, Aix-Marseille University, Marseille, France; Research Unit of Emerging Infectious and Tropical Diseases (URMITE) UMR CNRS, 7278 IRD 198 INSERM 1095, Institute of Research for the Development, Dakar, Senegal.
Ticks Tick Borne Dis. 2015 Mar;6(2):198-203. doi: 10.1016/j.ttbdis.2014.12.007. Epub 2015 Jan 10.
Ehrlichia canis and Anaplasma platys are bacteria belonging to the Anaplasmataceae family that cause acute, self-limiting and sometimes fatal vector-borne infections in dogs. These bacteria have been reported worldwide and are transmitted mainly by Rhipicephalus sanguineus. Aside from a report on E. canis once in 1935, no other Anaplasmataceae bacteria have been reported in Algeria to date. The aim of this study was to identify the microbial species implicated in ehrlichiosis and anaplasmosis by a molecular epidemiological survey in dogs. The study was carried out in Kabylie, in northeast Algeria. Sampling was performed in 11 municipalities in the province of Tizi Ouzou and 2 municipalities in the province of Béjaïa. Peripheral blood samples from 110 dogs were screened by qPCR, which is capable of identifying most Anaplasmataceae bacteria. Out of 110, a total of 13 samples screened positive (7/110 E. canis and 6/110 A. platys), and two genetic variants of A. platys and one of E. canis were identified. This is the first study to report the presence of A. platys in dogs from Algeria using a molecular investigative method. This survey was conducted in early spring. As tick activity can affect the prevalence of these pathogens in dogs, further investigations are needed to establish the year-round prevalence of these infections.
犬埃立克体和扁平无形体是属于无形体科的细菌，可引起犬类急性、自限性且有时致命的媒介传播感染。这些细菌在全球范围内均有报道，主要通过血红扇头蜱传播。除了1935年曾有过一次关于犬埃立克体的报道外，迄今为止阿尔及利亚尚未有其他无形体科细菌的报道。本研究的目的是通过对犬类进行分子流行病学调查，确定与埃立克体病和无形体病相关的微生物种类。该研究在阿尔及利亚东北部的卡比利亚地区进行。采样在提济乌祖省的11个市和贝贾亚省的2个市进行。通过能够识别大多数无形体科细菌的定量聚合酶链反应（qPCR）对110只犬的外周血样本进行筛查。在110份样本中，共有13份筛查呈阳性（7份为犬埃立克体阳性，6份为扁平无形体阳性），并鉴定出了扁平无形体的两个基因变体和犬埃立克体的一个基因变体。这是第一项使用分子调查方法报道阿尔及利亚犬类中存在扁平无形体的研究。这项调查是在早春进行的。由于蜱虫活动会影响这些病原体在犬类中的流行情况，因此需要进一步调查以确定这些感染的全年流行情况。
