Tamaoki J, Kanemura T, Kobayashi K, Sakai N, Takizawa T
First Department of Medicine, Tokyo Women's Medical College, Japan.
Peptides. 1989 Sep-Oct;10(5):1007-11. doi: 10.1016/0196-9781(89)90183-6.
We studied the effects of calcitonin gene-related peptide (CGRP) on ciliary beat frequency (CBF) and electrical properties of canine tracheal epithelium by a photoelectric method and Ussing's short-circuit technique, respectively. CGRP dose dependently increased CBF, an effect that was accompanied by elevation of intracellular cyclic AMP but not affected by blockade of either Ca2+-influx or arachidonic acid metabolism. In contrast, CGRP elicited only a small and transient increase in short-circuit current without significant alterations in transepithelial potential difference or tissue conductance. These results suggest that CGRP may play a role in regulating airway mucociliary transport function.
我们分别通过光电法和乌斯（Ussing）短路技术研究了降钙素基因相关肽（CGRP）对犬气管上皮细胞纤毛摆动频率（CBF）和电特性的影响。CGRP呈剂量依赖性增加CBF，这一效应伴随着细胞内环磷酸腺苷（cAMP）升高，但不受钙内流阻断或花生四烯酸代谢阻断的影响。相比之下，CGRP仅引起短路电流的小幅短暂增加，而跨上皮电位差或组织电导无明显改变。这些结果表明，CGRP可能在调节气道黏液纤毛转运功能中发挥作用。
