Maiorka Paulo Cesar, Favaron Phelipe Oliveira, Mess Andrea Maria, dos Santos Caio Rodrigues, Alberto Miryan Lanca, Meirelles Flavio Vieira, Miglino Maria Angelica
Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
Department of Surgery, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
PLoS One. 2015 Jan 13;10(1):e0106663. doi: 10.1371/journal.pone.0106663. eCollection 2015.
Although assisted reproductive techniques are commonly applied in humans and animals, they are frequently associated with major developmental deficits and reduced viability. To explore abnormalities associated with cloning or nuclear transfer (NT) as the most invasive of these methods, we used a bovine model to characterize abnormalities. Detailed necropsy examinations were done on 13 calves that died soon after birth; in addition, we included data from embryos and fetuses (produced by NT) that terminated prematurely. Bovine clones that survived until the neonatal period differed quantitatively and qualitatively from in-vivo-derived cattle. Although alterations affected a variety of organs (e.g. heart, lung and liver), there was a clear association with abberant vascular developmental during the early intrauterine phase. Therefore, we concluded that vascular problems were key alterations induced by cloning (presumably via epigenetic modifications).
尽管辅助生殖技术在人类和动物中普遍应用，但它们常常与严重的发育缺陷和生存能力降低相关。为了探究与克隆或核移植（NT）相关的异常情况（这些方法中最具侵入性的），我们使用牛模型来表征异常情况。对13头出生后不久死亡的犊牛进行了详细的尸检；此外，我们纳入了来自过早终止发育的胚胎和胎儿（由核移植产生）的数据。存活至新生儿期的牛克隆体在数量和质量上与体内衍生的牛不同。尽管改变影响了多种器官（如心脏、肺和肝脏），但在子宫内早期阶段与异常的血管发育存在明显关联。因此，我们得出结论，血管问题是克隆诱导的关键改变（可能通过表观遗传修饰）。
