Matsubayashi Hideaki, Kuruma Yutetsu, Ueda Takuya
Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba, 277-8562, Japan.
Orig Life Evol Biosph. 2014 Dec;44(4):331-4. doi: 10.1007/s11084-014-9389-y.
The cell membrane has many indispensable functions for sustaining cell alive besides a role as merely outer envelope. The most of such functions are implemented by membrane embedded proteins that are emerged through the membrane integration machinery, SecYEG translocon. Here, we synthesized SecYEG by expressing the corresponding gene in vitro to study the process of functionalization of the cell membrane.
细胞膜除了作为单纯的外层包膜外，对于维持细胞存活还有许多不可或缺的功能。这些功能大多是由通过膜整合机制SecYEG转运体嵌入膜中的蛋白质来实现的。在这里，我们通过在体外表达相应基因来合成SecYEG，以研究细胞膜功能化的过程。
