Maeda Yusuke T, Nakadai Tomoyoshi, Shin Jonghyeon, Uryu Kunihiro, Noireaux Vincent, Libchaber Albert
ACS Synth Biol. 2012 Feb 17;1(2):53-9. doi: 10.1021/sb200003v. Epub 2011 Dec 7.
The physical interaction between the cytoskeleton and the cell membrane is essential in defining the morphology of living organisms. In this study, we use a synthetic approach to polymerize bacterial MreB filaments inside phospholipid vesicles. When the proteins MreB and MreC are expressed inside the liposomes, the MreB cytoskeleton structure develops at the inner membrane. Furthermore, when purified MreB is used inside the liposomes, MreB filaments form a 4-10 μm rigid bundle structure and deform the lipid vesicles in physical contact with the vesicle inner membrane. These results indicate that the fibrillation of MreB filaments can take place either in close proximity of deformable lipid membrane or in the presence of associated protein. Our finding might be relevant for the self-assembly of cytoskeleton filaments toward the construction of artificial cell systems.
细胞骨架与细胞膜之间的物理相互作用对于确定生物体的形态至关重要。在本研究中，我们采用一种合成方法在磷脂囊泡内聚合细菌MreB细丝。当MreB和MreC蛋白在脂质体内表达时，MreB细胞骨架结构在内膜处形成。此外，当在脂质体内使用纯化的MreB时，MreB细丝形成4 - 10μm的刚性束状结构，并使与囊泡内膜物理接触的脂质囊泡变形。这些结果表明，MreB细丝的纤维化可以在可变形脂质膜附近或在相关蛋白存在的情况下发生。我们的发现可能与细胞骨架细丝的自组装以构建人工细胞系统有关。
