Herpesvirus in the oral cavity of children with leukaemia and its impact on the oral bacterial community profile.

作者信息

Bezerra Tacíria M, Ferreira Dennis C, Carmo Flávia L, Pinheiro Raquel, Leite Deborah C A, Cavalcante Fernanda S, Belinho Raquel A, Peixoto Raquel S, Rosado Alexandre S, dos Santos Kátia R N, Castro Gloria F B A

机构信息

Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil Department of Oral Medicine, School of Dentistry, CEUMA University, São Luis, MA, Brazil.

Institute of Microbiology Prof Paulo Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil Fellow by CAPES (Bolsista da CAPES)-Proc. n° BEX 9203-CAPES Foundation, Ministry of Education of Brazil, Brasilia/DF 70040-020, Brazil Department of Oral Medicine, School of Dentistry, Veiga de Almeida University, Rio de Janeiro, Brazil.

出版信息

J Clin Pathol. 2015 Mar;68(3):222-8. doi: 10.1136/jclinpath-2014-202668. Epub 2015 Jan 13.

DOI:10.1136/jclinpath-2014-202668

PMID:25586380

Abstract

AIMS

This cross-sectional study investigated the association between eight herpesviruses and the bacterial community profiles from the oral cavity of children with and without leukaemia.

METHODS

Sixty participants (aged 3-13), divided into the leukaemia group (LG) and healthy group (HG), were evaluated. Collection of medical data, intraoral examination and collection of clinical specimens were carried out. Single PCR and nested-PCR techniques were used to identify the viral types; denaturing gradient gel electrophoresis (DGGE) and real-time PCR techniques were used to evaluate the profile and abundance of bacterial communities.

RESULTS

All the children with leukaemia were positive for at least one type of herpesvirus, compared with healthy participants (33.3%; p<0.000). Human cytomegalovirus (HCMV; 46.7%), human herpesvirus-7 (HHV-7; 20%) and HHV-8 (77.3%) were in higher prevalence in the LG (p ≤ 0.01). Children with leukaemia had positive associations with the presence of HCMV, HHV-7 and HHV-8 in the oral cavity when under chemotherapy (p<0.05). There was a qualitative (means of DGGE bands) and quantitative (means of 16S rRNA gene abundance) difference in relation to the bacterial community between the two groups (p<0.05).

CONCLUSIONS

Based on the results, the prevalence of herpesviruses and the qualitative bacterial profiles was higher in children with leukaemia and HCMV, HHV-7 and HHV-8 were related to the use of chemotherapy. Moreover, HHV-6 was correlated with an increased bacterial community profile in patients with leukaemia (p<0.05). More attention should be paid to the oral health of these individuals, mainly those under chemotherapy, in order to prevent infections by opportunistic pathogens.

摘要