Potekhin S, Pfeil W
Institute of Protein Research, U.S.S.R. Academy of Sciences, Moscow Region.
Biophys Chem. 1989 Sep 15;34(1):55-62. doi: 10.1016/0301-4622(89)80041-9.
The conformational transitions of ferricytochrome c in acidic solutions with different NaCl concentrations have been studied by scanning and isothermal microcalorimetry. It is shown that ferricytochrome c adopts three different forms which are realized under the considered conditions: native, denatured (unfolded) and a compact native-like form with unique tertiary structure. The thermodynamic parameters of the corresponding transitions have been measured and the changes in the number of bound ligands (H+ and Cl-), accompanying these transitions, have been determined by analyzing the temperature, pH and ionic strength dependence of these parameters.
通过扫描量热法和等温微量热法研究了在不同NaCl浓度的酸性溶液中高铁细胞色素c的构象转变。结果表明，高铁细胞色素c呈现出三种不同的形式，它们在考虑的条件下实现：天然态、变性（展开）态和具有独特三级结构的紧密类天然态。测量了相应转变的热力学参数，并通过分析这些参数对温度、pH和离子强度的依赖性，确定了伴随这些转变的结合配体（H⁺和Cl⁻）数量的变化。
