Constantine Ryan S, Harrison Bridget, Davis Kathryn E, Rohrich Rod J
Department of Plastic and Reconstructive Surgery, University of Texas Southwestern Medical Center, Dallas, Tex.
Plast Reconstr Surg Glob Open. 2015 Jan 8;2(12):e260. doi: 10.1097/GOX.0000000000000228. eCollection 2014 Dec.
Fat grafting has been increasingly utilized in both aesthetic and reconstructive surgical procedures, yet the basic scientific understanding of fat grafting has lagged behind the pace of clinical innovation and utilization. This lack of basic scientific understanding has perhaps manifested itself in the wide range of graft viability reported across the literature. This study attempts to further the underlying mechanisms of fat graft take and viability through the comparison of the subcutaneous plane and the local fat pad in athymic rats.
Lipoaspirate from a consenting patient was grafted into 2 locations in the subcutaneous plane and into the 2 inguinal fat pads in each of 4 athymic rats. Specimens were then collected after 47 days, and immunohistochemistry was utilized to determine angiogenesis in the fat grafts as a measure of fat graft take. Data were analyzed using the Student's t test and analysis of variance followed by multiple comparisons.
There was no statistically significant difference (P = 0.2913) between the inguinal fat pad and the subcutaneous plane when measuring neovascularization. Analysis of variance comparing the graft locations also indicated no statistically significant difference when comparing each of the rats.
Investigation into fat graft injection location indicates that there is no statistically significant difference in angiogenesis signals between the subcutaneous plane and the local fat pad in the athymic rat model. Further research should aim to continue to close the gap between clinical practice and basic scientific understanding of fat grafting.
脂肪移植已越来越多地应用于美容和重建外科手术中，然而，对脂肪移植的基础科学认识却落后于临床创新和应用的步伐。这种基础科学认识的不足可能体现在文献中报道的广泛的移植存活率上。本研究试图通过比较无胸腺大鼠的皮下平面和局部脂肪垫，进一步了解脂肪移植存活和存活的潜在机制。
将一名同意参与研究的患者的脂肪抽吸物移植到4只无胸腺大鼠的皮下平面的2个部位和2个腹股沟脂肪垫中。47天后收集标本，采用免疫组织化学法测定脂肪移植中的血管生成，作为脂肪移植存活的指标。数据采用Student's t检验和方差分析，然后进行多重比较。
测量新生血管形成时，腹股沟脂肪垫和皮下平面之间无统计学显著差异（P = 0.2913）。比较移植部位的方差分析也表明，比较每只大鼠时无统计学显著差异。
对脂肪移植注射部位的研究表明，在无胸腺大鼠模型中，皮下平面和局部脂肪垫之间的血管生成信号无统计学显著差异。进一步的研究应旨在继续缩小临床实践与脂肪移植基础科学认识之间的差距。
