Shi Yan, Yuan Yi, Dong Ziqing, Gao Jianhua, Lu Feng
Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Dermatol Surg. 2016 Apr;42(4):535-42. doi: 10.1097/DSS.0000000000000615.
Although fat is transplanted into several layers, including subcutaneous, fat, and muscle layers, there is little (clinical) scientific basis for these procedures.
To determine the optimal recipient layer for fat transplantation.
Fat harvested from inguinal fat pads of green fluorescent protein (GFP) mice was grafted into the subcutaneous and intramuscular planes and the fat pads of C57 mice. Specimens collected after 1, 4, 8, 12, and 16 weeks were stained with hematoxylin and eosin to determine angiogenesis and fibrosis in the grafts. The survival rate of donor adipose tissue was determined by measuring GFP expression.
Fat was retained longer in fat pads than in subcutaneous layers of recipient mice and longer in subcutaneous than in intramuscular layers. Angiogenesis and vascularized connective tissue were greater in intramuscular than in subcutaneous or fat grafts. Neovascularization, however, was similar in fat pads and subcutaneous grafts. Survival rate was higher for intramuscularly injected fat than subcutaneously and fat pad injected fat.
Fat pad injection showed the highest graft retention rate, indicating that fat pads maybe the optimal area for fat transplantation. The increased blood supply to muscle suggests that intramuscular injection maybe optimal when there is little movement.
尽管脂肪被移植到包括皮下、脂肪和肌肉层在内的多个层次，但这些操作几乎没有（临床）科学依据。
确定脂肪移植的最佳受体层。
从绿色荧光蛋白（GFP）小鼠腹股沟脂肪垫采集的脂肪被移植到C57小鼠的皮下、肌内平面和脂肪垫。在1、4、8、12和16周后采集的标本用苏木精和伊红染色，以确定移植物中的血管生成和纤维化情况。通过测量GFP表达来确定供体脂肪组织的存活率。
脂肪在受体小鼠脂肪垫中的保留时间比在皮下层更长，在皮下层中的保留时间比在肌内层更长。肌内移植物中的血管生成和血管化结缔组织比皮下或脂肪移植物中的更多。然而，脂肪垫和皮下移植物中的新生血管形成相似。肌内注射脂肪的存活率高于皮下和脂肪垫注射脂肪。
脂肪垫注射显示出最高的移植物保留率，表明脂肪垫可能是脂肪移植的最佳区域。肌肉血液供应的增加表明，在几乎没有运动的情况下，肌内注射可能是最佳选择。
