Lane Selena L, Koenig Amie, Brainard Benjamin M
Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602.
J Am Vet Med Assoc. 2015 Feb 1;246(3):307-12. doi: 10.2460/javma.246.3.307.
To determine the effect of PCV on veterinary point-of-care (POC) glucometer measurements in canine blood samples and develop a formula to correct the glucose concentration as measured by a point-of-care glucometer (POCgluc) given a known PCV.
Experimental and prospective study.
Blood samples from 6 healthy dogs and from 30 hospitalized dogs.
60 mL of heparinized blood was obtained from each of 6 healthy dogs. Samples were processed into packed RBCs and plasma. Packed RBCs were resuspended with plasma to achieve a range of PCVs from 0% to 94%. Duplicate POCgluc and PCV measurements were obtained for each dilution; following POCgluc measurements, plasma samples were analyzed for glucose concentration by a clinical laboratory biochemical analyzer (LABgluc). A correction formula for POCgluc was developed. Measurements of POCgluc, PCV, and LABgluc were also determined from blood samples of 30 dogs admitted to the veterinary teaching hospital.
Values of LABgluc for each sample were similar at any PCV. As PCV decreased, POCgluc was falsely increased; as PCV increased, POCgluc was falsely decreased, compared with LABgluc. The absolute difference between POCgluc and LABgluc increased as the PCV changed from 50%. Compared with POCgluc, the corrected POCgluc had a significantly improved correlation with LABgluc, which was also reflected in improvements in Clarke and consensus error grid analyses.
Results indicated that in dogs with hemodilution or hemoconcentration, POCgluc did not reflect actual patient glucose concentrations. Use of a correction formula reduced this error. Corrected POCgluc data had strong, significant correlations with LABgluc data.
确定红细胞压积（PCV）对犬类血液样本中兽医即时检测（POC）血糖仪测量值的影响，并制定一个公式，用于在已知PCV的情况下校正即时检测血糖仪（POCgluc）所测的葡萄糖浓度。
实验性前瞻性研究。
来自6只健康犬和30只住院犬的血液样本。
从6只健康犬中每只获取60 mL肝素化血液。样本被处理成压积红细胞和血浆。将压积红细胞与血浆重悬，以达到0%至94%的一系列PCV值。对每个稀释度进行重复的POCgluc和PCV测量；在进行POCgluc测量后，通过临床实验室生化分析仪（LABgluc）分析血浆样本中的葡萄糖浓度。制定了POCgluc的校正公式。还从兽医教学医院收治的30只犬的血液样本中测定了POCgluc、PCV和LABgluc。
在任何PCV下，每个样本的LABgluc值相似。与LABgluc相比，随着PCV降低，POCgluc被错误地升高；随着PCV升高，POCgluc被错误地降低。当PCV从50%变化时，POCgluc与LABgluc之间的绝对差值增大。与POCgluc相比，校正后的POCgluc与LABgluc的相关性显著改善，这也反映在克拉克误差分析和一致性误差网格分析的改善上。
结果表明，在有血液稀释或血液浓缩的犬中，POCgluc不能反映患者的实际葡萄糖浓度。使用校正公式可减少这种误差。校正后的POCgluc数据与LABgluc数据有很强的显著相关性。
