Proença Laila M, Camus Melinda, Nemeth Nicole, Sharma Ajay, Stelmach Dainna, Mayer Jörg, Divers Stephen J
Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Georgia, Athens, GA 30602.
J Am Vet Med Assoc. 2015 Feb 1;246(3):313-20. doi: 10.2460/javma.246.3.313.
To evaluate diagnostic quality of liver percutaneous ultrasound-guided fine-needle aspirates and laparoscopic biopsy specimens of rabbits (Oryctolagus cuniculus).
Prospective descriptive study.
7 healthy adult rabbits.
3 to 5 liver fine-needle aspirates were obtained with a 22-gauge needle under ultrasound guidance in anesthetized rabbits. Liver biopsy specimens were also obtained with 1.7-mm (n = 2) or 3.0-mm (1) biopsy forceps by direct laparoscopic observation. Fine-needle aspirates were cytologically evaluated on a scale from 0 (suboptimal specimen) to 3 (optimal specimen) for cellularity, cell distribution, cell preservation, cell morphology, and blood contamination. Biopsy specimens were histologically evaluated on a scale from 0 (optimal specimen) to 5 (suboptimal specimen) for artifactual changes; numbers of portal triads and central veins were quantified.
Aspirates were moderately to highly cellular (mean, 2.54) with good cell distribution (mean, 2.56), good cell preservation (mean, 2.20), and moderate blood contamination (mean, 1.04). The 1.7-mm biopsy specimens had a mean score of 1.3 for artifactual changes and contained a mean of 0.6 portal triads and 1.6 central veins/biopsy specimen. The 3.0-mm liver biopsy specimens had a mean score of 2.7 for artifactual changes, with a mean of 4.0 portal triads and 4.14 central veins/biopsy specimen. All but one 3.0-mm liver biopsy specimen had ≥ 1 portal triad suitable for histologic evaluation, and all had ≥ 1 central vein; in contrast, only half of the 1.7-mm liver biopsy specimens had a discernible portal triad or central vein.
For histologic evaluation, advantages of obtaining 3.0-mm liver biopsy specimens, compared with 1.7-mm liver biopsy specimens or fine-needle aspirates, should be considered in rabbits with suspected liver disease.
评估兔（穴兔）经皮肝脏超声引导下细针穿刺抽吸物及腹腔镜活检标本的诊断质量。
前瞻性描述性研究。
7只健康成年兔。
在麻醉的兔身上，于超声引导下用22号针获取3至5份肝脏细针穿刺抽吸物。还通过直接腹腔镜观察，用1.7毫米（n = 2）或3.0毫米（n = 1）活检钳获取肝脏活检标本。对细针穿刺抽吸物进行细胞学评估，从0（欠佳标本）到3（最佳标本），评估细胞数量、细胞分布、细胞保存、细胞形态和血液污染情况。对活检标本进行组织学评估，从0（最佳标本）到5（欠佳标本），评估人为改变情况；对门三联管和中央静脉数量进行量化。
穿刺抽吸物细胞数量为中度至高度（平均2.54），细胞分布良好（平均2.56），细胞保存良好（平均2.20），血液污染中度（平均1.04）。1.7毫米活检标本人为改变平均评分为1.3，每份活检标本平均含0.6个门三联管和1.6个中央静脉。3.0毫米肝脏活检标本人为改变平均评分为2.7，每份活检标本平均含4.0个门三联管和4.14个中央静脉。除1份3.0毫米肝脏活检标本外，其余所有标本均有≥1个适合组织学评估的门三联管，且均有≥1个中央静脉；相比之下，仅一半的1.7毫米肝脏活检标本有可辨认的门三联管或中央静脉。
对于组织学评估，在怀疑有肝脏疾病的兔中，与1.7毫米肝脏活检标本或细针穿刺抽吸物相比，获取3.0毫米肝脏活检标本的优势应予以考虑。
