Vaxevanidou K, Christou C, Kremmydas G F, Georgakopoulos D G, Papassiopi N
Laboratory of Metallurgy, School of Mining and Metallurgical Engineering, National Technical University of Athens, Zografos Campus, 15780, Athens, Greece.
Bull Environ Contam Toxicol. 2015 Mar;94(3):282-8. doi: 10.1007/s00128-015-1458-z. Epub 2015 Jan 15.
In this study two different treatment options were investigated for the release of arsenic from a contaminated soil sample. The first option was based on the "bioaugmentation" principle and involved addition of a pure Fe(III)-reducing culture, i.e. Desulfuromonas palmitatis. The second option consisted in the "biostimulation" of indigenous bacteria and involved simple addition of nutrients. Due to the strong association of As with soil ferric oxides, the reductive dissolution of soil oxides by D. palmitatis lead to 45 % arsenic release in solution (2.15 mM). When only nutrients were supplied to the soil, the same amounts of Fe and As were dissolved with slower rates and most aqueous As was found to be in the trivalent state, indicating the presence of arsenate reducing species. The arsenate reducing microorganisms were enriched with successive cultures, using Na2HAsO4 as electron acceptor. The phylogenetic analysis revealed that the enriched microbial consortium contained Desulfosporosinus species, which are known arsenate reducers.
在本研究中，对从受污染土壤样品中释放砷的两种不同处理方案进行了研究。第一种方案基于“生物强化”原理，涉及添加纯的铁（III）还原培养物，即棕榈酸脱硫单胞菌。第二种方案在于对本地细菌进行“生物刺激”，仅涉及简单添加营养物质。由于砷与土壤铁氧化物的强烈结合，棕榈酸脱硫单胞菌对土壤氧化物的还原溶解导致溶液中45%的砷释放（2.15 mM）。当仅向土壤供应营养物质时，相同量的铁和砷以较慢的速率溶解，并且发现大多数水溶态砷处于三价状态，表明存在砷酸盐还原菌。利用Na2HAsO4作为电子受体，通过连续培养富集砷酸盐还原微生物。系统发育分析表明，富集的微生物群落包含脱硫孢菌属物种，它们是已知的砷酸盐还原菌。
