Moens P B, Earnshaw W C
Department of Biology, York University, Ontario, Canada.
Chromosoma. 1989 Nov;98(5):317-22. doi: 10.1007/BF00292383.
At meiotic prophase the chromatin becomes arranged in loops on newly formed chromosome cores. The cores of homologous chromosomes become aligned in parallel and thus form the synaptonemal complex (SC), a structure found in the meiocytes of nearly all recombinationally competent, sexually reproducing organisms. We report that two polyclonal antibodies against topoisomerase II (topo II), which recognize the mitotic metaphase chromosome scaffold give, at pachytene, a positive immunocytological reaction with the chromatin and, predominantly, with the cores and centromeric regions of the paired chromosomes. It therefore appears that during meiotic prophase, topo II - a DNA-binding enzyme implicated in transient double-strand breaks, chromosome condensation, and anaphase separation - is associated with the chromatin and SCs of the pachytene and diplotene chromosomes.
在减数分裂前期，染色质在新形成的染色体核心上排列成环。同源染色体的核心平行排列，从而形成联会复合体（SC），这是一种在几乎所有具有重组能力的有性生殖生物体的减数分裂细胞中都能发现的结构。我们报告，两种针对拓扑异构酶II（topo II）的多克隆抗体，它们识别有丝分裂中期染色体支架，在粗线期对染色质产生阳性免疫细胞反应，主要是与配对染色体的核心和着丝粒区域产生反应。因此，在减数分裂前期，拓扑异构酶II（一种与瞬时双链断裂、染色体浓缩和后期分离有关的DNA结合酶）似乎与粗线期和双线期染色体的染色质和联会复合体相关。
