Heyting C, Moens P B, van Raamsdonk W, Dietrich A J, Vink A C, Redeker E J
Eur J Cell Biol. 1987 Feb;43(1):148-54.
This paper describes the identification of two major components of the lateral elements of synaptonemal complexes of the rat by immunocytochemical techniques. We prepared monoclonal antibodies against synaptonemal complexes (SCs) by immunization of mice with purified SCs. One of these antibodies, II52F10, reacts with a 30 and a 33 kDa polypeptide, which are major components of purified SCs. Using this antibody, we studied the localization of its antigens light microscopically, by means of the indirect immunoperoxidase technique, as well as ultrastructurally, by means of the immunogold labeling technique. The immunolocalization was carried out on whole-mount preparations of lysed spermatocytes. The antibody reacts with paired as well as unpaired segments of zygotene, pachytene and diplotene SCs. In light microscopic preparations, the attachment plaques, particularly those of late pachytene and diplotene SCs, also appear to react strongly. In electron micrographs the lateral elements in paired as well as unpaired segments could be seen to react. No reaction was observed in the attachment plaques; however, in late pachytene and diplotene SCs the swollen terminal segments of the lateral elements did react with the antibody. Thus, we conclude that a 30 and a 33 kDa polypeptide make part of the lateral elements of synaptonemal complexes of the rat.
本文描述了用免疫细胞化学技术鉴定大鼠联会复合体侧生元件的两个主要成分。我们用纯化的联会复合体（SCs）免疫小鼠，制备了针对联会复合体的单克隆抗体。其中一种抗体II52F10与30 kDa和33 kDa的多肽发生反应，这两种多肽是纯化SCs的主要成分。利用该抗体，我们通过间接免疫过氧化物酶技术在光学显微镜下以及通过免疫金标记技术在超微结构水平上研究了其抗原的定位。免疫定位是在裂解的精母细胞整装制片上进行的。该抗体与偶线期、粗线期和双线期SCs的配对及未配对片段都发生反应。在光学显微镜制片中，附着斑，尤其是粗线期末期和双线期SCs的附着斑，似乎也有强烈反应。在电子显微照片中，可以看到配对及未配对片段中的侧生元件发生反应。附着斑未观察到反应；然而，在粗线期末期和双线期SCs中，侧生元件肿胀的末端片段确实与该抗体发生反应。因此，我们得出结论，30 kDa和33 kDa的多肽是大鼠联会复合体侧生元件的组成部分。
