Guo Peng, Zeng Jing-jing, Zhou Nuo
College of Stomatology, GuangXi Mediceal University, Nanning Guangxi, China.
Scanning. 2015 Jan-Feb;37(1):42-8. doi: 10.1002/sca.21177. Epub 2015 Jan 14.
The aim of this study was to fabricate canine bone marrow mesenchymal stem cell sheet through the use of vitamin C, to identify the biological characteristics of the resulting cell sheets, and to reveal the potential mechanism of cell-sheet promotion by vitamin C. This study used vitamin C to induce bone marrow mesenchymal stem cells to proliferate. The resulting cells secreted large amounts of collagen, thereby shortening the construction time of the cell-sheet layer. In addition to these aims, we identified biological microcharacteristics of the cell sheet through histological observation, transmission electron microscopy, real-time PCR detection, immunohistochemical detection, and osteogenesis-induction experiments on the cell sheet. We were able to stably and rapidly construct bone marrow mesenchymal stem cell sheet, effectively harvest it, and transfer the seed cells for tissue engineering. This study indicates that the use of vitamin C for harvesting mesenchymal stem cell sheets from bone marrow may provide an easy and practical approach for bone tissue regeneration.
本研究旨在通过使用维生素C制备犬骨髓间充质干细胞片，鉴定所得细胞片的生物学特性，并揭示维生素C促进细胞片形成的潜在机制。本研究使用维生素C诱导骨髓间充质干细胞增殖。所得细胞分泌大量胶原蛋白，从而缩短了细胞片层的构建时间。除了这些目标外，我们还通过组织学观察、透射电子显微镜、实时PCR检测、免疫组织化学检测以及对细胞片进行成骨诱导实验，鉴定了细胞片的生物学微观特征。我们能够稳定、快速地构建骨髓间充质干细胞片，有效地收获它，并将种子细胞用于组织工程。本研究表明，使用维生素C从骨髓中收获间充质干细胞片可能为骨组织再生提供一种简便实用的方法。
