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层粘连蛋白-521在光诱导细胞片技术上促进大鼠骨髓间充质干细胞片的形成。

Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology.

作者信息

Jiang Zhiwei, Xi Yue, Lai Kaichen, Wang Ying, Wang Huiming, Yang Guoli

机构信息

Department of Implantology, Stomatology Hospital, School of Medicine, Zhejiang University, Yan'an Road, Hangzhou, China.

Department of Oral Medicine, Stomatology Hospital, School of Medicine, Zhejiang University, Yan'an Road, Hangzhou, China.

出版信息

Biomed Res Int. 2017;2017:9474573. doi: 10.1155/2017/9474573. Epub 2017 Jan 9.

DOI:10.1155/2017/9474573
PMID:28164129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5253502/
Abstract

Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering. However, methods of culturing rBMSC sheets are critically limited. In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study. TiO nanodot films were coated with (TL) or without (TN) laminin-521. We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing. The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential. The results showed that laminin-521 could promote the formation of rBMSC sheets with good viability under hyperconfluent conditions. Cell sheet thickness increased from an initial 26.7 ± 1.5 m (day 5) up to 47.7 ± 3.0 m (day 10). Moreover, rBMSC sheets maintained their potential of osteogenic, adipogenic, and chondrogenic differentiation. This study provides a new strategy to obtain rBMSC sheets using light-induced cell sheet technology.

摘要

大鼠骨髓间充质干细胞片层（rBMSC片层）在基于细胞的组织工程中具有吸引力。然而，rBMSC片层的培养方法极为有限。为了获得完整的rBMSC片层，本研究采用了光诱导细胞片层法。TiO纳米点膜涂有（TL）或未涂有（TN）层粘连蛋白-521。我们研究了层粘连蛋白-521在细胞片层培养过程中对rBMSCs的影响。随后对制备的rBMSC片层进行评估，以研究细胞片层的活力、重新附着能力、细胞片层厚度、I型胶原蛋白沉积和多向分化潜能。结果表明，层粘连蛋白-521可在高汇合条件下促进具有良好活力的rBMSC片层的形成。细胞片层厚度从最初的26.7±1.5μm（第5天）增加到47.7±3.0μm（第10天）。此外，rBMSC片层保持了其成骨、成脂和成软骨分化的潜能。本研究提供了一种利用光诱导细胞片层技术获得rBMSC片层的新策略。

相似文献

[1]
Laminin-521 Promotes Rat Bone Marrow Mesenchymal Stem Cell Sheet Formation on Light-Induced Cell Sheet Technology.

Biomed Res Int. 2017

[2]
Multilineage potential of bone-marrow-derived mesenchymal stem cell cell sheets: implications for tissue engineering.

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[3]
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[4]
[Construction of three-dimensional dermoid tissue based on cell sheets technology ].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2020-1-15

[5]
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Tissue Eng. 2004

[6]
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[7]
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[8]
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[9]
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Stem Cell Res Ther. 2015-12-21

[10]
Different tenogenic differentiation capacities of different mesenchymal stem cells in the presence of BMP-12.

J Transl Med. 2015-6-24

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[2]
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Biomed Eng Online. 2023-2-9

[3]
Cell Sheet Technology as an Engineering-Based Approach to Bone Regeneration.

Int J Nanomedicine. 2022

[4]
Therapeutic potential of dental pulp stem cells and their derivatives: Insights from basic research toward clinical applications.

World J Stem Cells. 2022-7-26

[5]
Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment.

Sci Rep. 2020-7-24

[6]
Regulation of body length and bone mass by Gpr126/Adgrg6.

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[7]
Recent advances in cell sheet technology for bone and cartilage regeneration: from preparation to application.

Int J Oral Sci. 2019-5-21

[8]
The legacy effects of electromagnetic fields on bone marrow mesenchymal stem cell self-renewal and multiple differentiation potential.

Stem Cell Res Ther. 2018-8-9

[9]
Extracellular matrix scaffold and hydrogel derived from decellularized and delipidized human pancreas.

Sci Rep. 2018-7-11

[10]
A Concise Review on the Use of Mesenchymal Stem Cells in Cell Sheet-Based Tissue Engineering with Special Emphasis on Bone Tissue Regeneration.

Stem Cells Int. 2017

本文引用的文献

[1]
Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke.

Stem Cells Int. 2016

[2]
Effects of RGD immobilization on light-induced cell sheet detachment from TiO2 nanodots films.

Mater Sci Eng C Mater Biol Appl. 2016-2-27

[3]
Higher-Density Culture in Human Embryonic Stem Cells Results in DNA Damage and Genome Instability.

Stem Cell Reports. 2016-3-8

[4]
Improved Human Pluripotent Stem Cell Attachment and Spreading on Xeno-Free Laminin-521-Coated Microcarriers Results in Efficient Growth in Agitated Cultures.

Biores Open Access. 2015-4-1

[5]
Improved light-induced cell detachment on rutile TiO₂ nanodot films.

Acta Biomater. 2015-10

[6]
α-5 Laminin Synthesized by Human Pluripotent Stem Cells Promotes Self-Renewal.

Stem Cell Reports. 2015-8-11

[7]
Influence of nanotopography on periodontal ligament stem cell functions and cell sheet based periodontal regeneration.

Int J Nanomedicine. 2015-6-19

[8]
CD49f Acts as an Inflammation Sensor to Regulate Differentiation, Adhesion, and Migration of Human Mesenchymal Stem Cells.

Stem Cells. 2015-9

[9]
Carrier-free cultured autologous oral mucosa epithelial cell sheet (CAOMECS) for corneal epithelium reconstruction: a histological study.

Ocul Surf. 2015-1-30

[10]
A novel in vitro method for detecting undifferentiated human pluripotent stem cells as impurities in cell therapy products using a highly efficient culture system.

PLoS One. 2014-10-27

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