Jiang Zhiwei, Xi Yue, Lai Kaichen, Wang Ying, Wang Huiming, Yang Guoli
Department of Implantology, Stomatology Hospital, School of Medicine, Zhejiang University, Yan'an Road, Hangzhou, China.
Department of Oral Medicine, Stomatology Hospital, School of Medicine, Zhejiang University, Yan'an Road, Hangzhou, China.
Biomed Res Int. 2017;2017:9474573. doi: 10.1155/2017/9474573. Epub 2017 Jan 9.
Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering. However, methods of culturing rBMSC sheets are critically limited. In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study. TiO nanodot films were coated with (TL) or without (TN) laminin-521. We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing. The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential. The results showed that laminin-521 could promote the formation of rBMSC sheets with good viability under hyperconfluent conditions. Cell sheet thickness increased from an initial 26.7 ± 1.5 m (day 5) up to 47.7 ± 3.0 m (day 10). Moreover, rBMSC sheets maintained their potential of osteogenic, adipogenic, and chondrogenic differentiation. This study provides a new strategy to obtain rBMSC sheets using light-induced cell sheet technology.
大鼠骨髓间充质干细胞片层（rBMSC片层）在基于细胞的组织工程中具有吸引力。然而，rBMSC片层的培养方法极为有限。为了获得完整的rBMSC片层，本研究采用了光诱导细胞片层法。TiO纳米点膜涂有（TL）或未涂有（TN）层粘连蛋白-521。我们研究了层粘连蛋白-521在细胞片层培养过程中对rBMSCs的影响。随后对制备的rBMSC片层进行评估，以研究细胞片层的活力、重新附着能力、细胞片层厚度、I型胶原蛋白沉积和多向分化潜能。结果表明，层粘连蛋白-521可在高汇合条件下促进具有良好活力的rBMSC片层的形成。细胞片层厚度从最初的26.7±1.5μm（第5天）增加到47.7±3.0μm（第10天）。此外，rBMSC片层保持了其成骨、成脂和成软骨分化的潜能。本研究提供了一种利用光诱导细胞片层技术获得rBMSC片层的新策略。
