百日咳毒素治疗可抵消神经肽 Y 受体与α2 肾上腺素能受体之间的膜内相互作用。

Pertussis toxin treatment counteracts intramembrane interactions between neuropeptide Y receptors and alpha 2-adrenoceptors.

作者信息

Von Euler G, Fuxe K, Van der Ploeg I, Fredholm B B, Agnati L F

机构信息

Department of Histology and Neurobiology, Karolinska Institute, Stockholm, Sweden.

出版信息

Eur J Pharmacol. 1989 Dec 5;172(6):435-41. doi: 10.1016/0922-4106(89)90026-6.

DOI:10.1016/0922-4106(89)90026-6

PMID:2558898

Abstract

The effect of intracerebroventricular injections of pertussis toxin were investigated on the neuropeptide Y-induced modulation of alpha 2-adrenoceptor binding in membranes from the dorsomedial medulla oblongata of the rat. Concentration-response experiments showed that neuropeptide Y reduced the binding affinity of the alpha 2-agonist, p-[3H]aminoclonidine, with a maximal effect of 30% at 3-30 nM. Pertussis toxin treatment (10 micrograms, 24 h) counteracted this modulation, without reducing the binding of neuropeptide Y to its own receptor. The results indicate that pertussis toxin-sensitive G-proteins are essential for the mediation of the intramembrane interaction between neuropeptide Y receptors and alpha 2-adrenoceptors.

摘要

研究了脑室内注射百日咳毒素对大鼠延髓背内侧膜中神经肽Y诱导的α2-肾上腺素能受体结合调节的影响。浓度-反应实验表明，神经肽Y降低了α2-激动剂p-[3H]氨基可乐定的结合亲和力，在3-30 nM时最大效应为30%。百日咳毒素处理（10微克，24小时）可抵消这种调节作用，而不会降低神经肽Y与其自身受体的结合。结果表明，百日咳毒素敏感的G蛋白对于介导神经肽Y受体与α2-肾上腺素能受体之间的膜内相互作用至关重要。