DNA促旋酶抑制剂使大肠杆菌生物降解性苏氨酸脱水酶的表达增加。
Increased expression of biodegradative threonine dehydratase of Escherichia coli by DNA gyrase inhibitors.
作者信息
Sumantran V N, Tranguch A J, Datta P
机构信息
Department of Biological Chemistry, University of Michigan, Ann Arbor 48109-0606.
出版信息
FEMS Microbiol Lett. 1989 Nov;53(1-2):37-40. doi: 10.1016/0378-1097(89)90362-5.
The synthesis of inducible biodegradative threonine dehydratase of Escherichia coli increased several-fold in the presence of the DNA gyrase inhibitors, nalidixic acid and coumermycin. Temperature-sensitive gyrB mutants expressed higher levels of dehydratase as compared to an isogenic gyrB+ strain. Immunoblotting experiments showed increased synthesis of the dehydratase protein in the presence of gyrase inhibitors; addition of rifampicin and chloramphenicol to cells actively synthesizing enzyme preventing new enzyme production. Increased expression of dehydratase by gyrase inhibitors was accompanied by relaxation of supercoiled DNA.
在DNA促旋酶抑制剂萘啶酸和香豆霉素存在的情况下，大肠杆菌诱导型生物降解苏氨酸脱水酶的合成增加了几倍。与同基因的gyrB+菌株相比，温度敏感型gyrB突变体表达出更高水平的脱水酶。免疫印迹实验表明，在促旋酶抑制剂存在的情况下，脱水酶蛋白的合成增加；向正在积极合成酶的细胞中添加利福平和氯霉素可阻止新酶的产生。促旋酶抑制剂导致脱水酶表达增加的同时，超螺旋DNA发生了松弛。
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