Barrenas Fredrik, Green Richard R, Thomas Matthew J, Law G Lynn, Proll Sean C, Engelmann Flora, Messaoudi Ilhem, Marzi Andrea, Feldmann Heinz, Katze Michael G
Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA.
Clin Vaccine Immunol. 2015 Mar;22(3):354-6. doi: 10.1128/CVI.00733-14. Epub 2015 Jan 14.
Vesicular stomatitis virus expressing Zaire Ebola virus (EBOV) glycoprotein (VSVΔG/EBOVgp) could be used as a vaccine to meet the 2014 Ebola virus outbreak. To characterize the host response to this vaccine, we used mRNA sequencing to analyze peripheral blood mononuclear cells (PBMCs) from cynomolgus macaques after VSVΔG/EBOVgp immunization and subsequent EBOV challenge. We found a controlled transcriptional response that transitioned to immune regulation as the EBOV was cleared. This observation supports the safety of the vaccine.
表达扎伊尔埃博拉病毒（EBOV）糖蛋白的水疱性口炎病毒（VSVΔG/EBOVgp）可作为一种疫苗用于应对2014年埃博拉病毒疫情。为了表征宿主对该疫苗的反应，我们利用mRNA测序分析了食蟹猴在接种VSVΔG/EBOVgp并随后接受埃博拉病毒攻击后外周血单个核细胞（PBMC）的情况。我们发现了一种可控的转录反应，随着埃博拉病毒被清除，该反应转变为免疫调节。这一观察结果支持了该疫苗的安全性。
