Kabiru Lawan Mohammed, Bello Mohammed, Kabir Junaid, Grande Laura, Morabito Stefano
Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, P.M.B. 1096, Zaria 2222, Nigeria.
EU Reference Laboratory for E. coli, Veterinary Public Health and Food Safety Department, Istituto Superiore di Sanita, Viale Regina Elena 299, Rome 00161, Italy.
Int J Environ Res Public Health. 2015 Jan 13;12(1):679-91. doi: 10.3390/ijerph120100679.
Pathogenic Escherichia coli can be released with the wastes coming from slaughterhouses into the environment, where they can persist. We investigated the presence of diarrheagenic E. coli in specimens taken at an abattoir located in the Zaria region, Nigeria, in samples of water from the river Koreye, where the effluent from the abattoir spills in, and vegetable specimens taken at a nearby farm. All the isolated E. coli were assayed for the production of Shiga toxins (Stx) by using the Ridascreen verotoxin Immunoassay and by PCR amplification of genes associated with the diarrheagenic E. coli. Three strains from the rectal content of two slaughtered animals and a cabbage were positive for the presence of the Stx-coding genes. Additionally we have isolated one Enteroaggregative E. coli (EAggEC) from the abattoir effluent and two Subtilase-producing E. coli from the slaughterhouse's effluent and a sample of carrots. Our results provide evidence that pathogenic E. coli can contaminate the environment as a result of the discharge into the environment of untreated abattoir effluent, representing a reservoir for STEC and other diarrheagenic E. coli favouring their spread to crops.
致病性大肠杆菌可随屠宰场废弃物排放到环境中，并在环境中持续存在。我们调查了尼日利亚扎里亚地区一家屠宰场采集的样本、屠宰场废水流入的科雷耶河中水样以及附近农场采集的蔬菜样本中致泻性大肠杆菌的存在情况。所有分离出的大肠杆菌都通过使用Ridascreen 维罗毒素免疫测定法以及对与致泻性大肠杆菌相关的基因进行PCR扩增来检测志贺毒素（Stx）的产生。来自两只屠宰动物直肠内容物和一棵卷心菜的三个菌株的志贺毒素编码基因呈阳性。此外，我们从屠宰场废水分离出一株肠聚集性大肠杆菌（EAggEC），从屠宰场废水和一份胡萝卜样本中分离出两株产枯草杆菌蛋白酶大肠杆菌。我们的结果表明，未经处理的屠宰场废水排放到环境中会导致致病性大肠杆菌污染环境，这是产志贺毒素大肠杆菌（STEC）和其他致泻性大肠杆菌的一个储存库，有利于它们传播到农作物。
