McMahon C A, Dock N L, Lentz E B, Forbes B A, Reinitz E R, Lamberson H V
Research & Development Laboratory, American Red Cross Blood Services, Syracuse NY 13202.
J Clin Lab Anal. 1989;3(6):350-4. doi: 10.1002/jcla.1860030607.
We have compared two IgM-specific cytomegalovirus (CMV) antibody assays, an immunofluorescence assay (IFA-M) and an enzyme-linked antigen immunoassay (ELA-M), with an assay for CMV total antibody (ELISA) and viral culture for the detection of active CMV infection in renal transplant recipients. Of 75 patients (49 ELISA negative pretransplant, 26 ELISA positive), CMV-specific IgM was detected in 35 (27 ELISA negative pretransplant, 8 ELISA positive) using the IFA-M assay and in 25 (16 ELISA negative pretransplant, 9 ELISA positive) using the ELA-M test. Of the 25 patients identified as positive by ELA-M, 21 had positive viral cultures post-transplant, two seronegative patients had evidence of infection indicated by post-transplant seroconversion, and two patients were seropositive pretransplant but remained viral culture negative throughout the follow-up period. ELA-M and CMV total antibody ELISA detected primary infection in renal transplant recipients equally well, but ELA-M was found to be superior to ELISA and IFA-M for detecting reinfection and reactivation infections.
我们比较了两种针对巨细胞病毒（CMV）IgM的抗体检测方法，即免疫荧光检测法（IFA-M）和酶联抗原免疫检测法（ELA-M），同时与CMV总抗体检测法（ELISA）以及病毒培养法进行比较，以检测肾移植受者中的活动性CMV感染。在75例患者中（49例移植前ELISA检测为阴性，26例移植前ELISA检测为阳性），使用IFA-M检测法在35例患者中检测到CMV特异性IgM（27例移植前ELISA检测为阴性，8例移植前ELISA检测为阳性），使用ELA-M检测法在25例患者中检测到CMV特异性IgM（16例移植前ELISA检测为阴性，9例移植前ELISA检测为阳性）。在ELA-M检测为阳性的25例患者中，21例移植后病毒培养呈阳性，2例血清阴性患者有移植后血清转化表明感染的证据，2例患者移植前血清学呈阳性但在整个随访期病毒培养均为阴性。ELA-M和CMV总抗体ELISA在检测肾移植受者的原发性感染方面效果相当，但发现ELA-M在检测再感染和再激活感染方面优于ELISA和IFA-M。
