用光生物素标记的双链DNA探针原位杂交快速检测单纯疱疹病毒DNA
Rapid detection of herpes simplex virus DNA by in situ hybridization with photobiotin-labelled double-stranded DNA probes.
作者信息
Chantratita W, Henchal E A, Yoosook C
机构信息
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
出版信息
Mol Cell Probes. 1989 Dec;3(4):363-73. doi: 10.1016/0890-8508(89)90015-7.
PMID:2559320
Abstract
An assay for rapid detection of herpes simplex virus in infected cells is described. The assay utilizes in situ hybridization with photobiotin-labelled double-stranded DNA probes prepared from HSV-1 DNA cloned in plasmid vectors. The assay provided an alternative method for earlier detection of virus in cell cultures with the ease of preparation of photobiotin-labelled double-stranded DNA.
摘要
本文描述了一种用于快速检测感染细胞中单纯疱疹病毒的检测方法。该检测方法利用了原位杂交技术，使用从克隆于质粒载体中的HSV-1 DNA制备的光生物素标记的双链DNA探针。该检测方法提供了一种替代方法，可通过轻松制备光生物素标记的双链DNA，在细胞培养物中更早地检测到病毒。
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