通过DNA杂交检测临床标本中的单纯疱疹病毒。

Detection of herpes simplex virus in clinical specimens by DNA hybridization.

作者信息

Redfield D C, Richman D D, Albanil S, Oxman M N, Wahl G M

出版信息

Diagn Microbiol Infect Dis. 1983 Jun;1(2):117-28. doi: 10.1016/0732-8893(83)90041-x.

Abstract

An assay to detect herpes simplex virus (HSV) DNA in clinical specimens has been developed. It utilizes nucleic acid hybridization with a 32P-labeled DNA probe prepared from a fragment of HSV DNA cloned in a plasmid vector. This assay can detect 5 X 10(4) plaque-forming units of cell-free HSV and as few as four virus-infected cells. The assay has a sensitivity of 78% and a specificity of 100% compared to virus culture for the detection of HSV in swab specimens from genital lesions. No hybridization is observed with uninfected, varicella-zoster virus infected, or cytomegalovirus infected cells, and specimens from herpes zoster lesions are uniformly negative. While hybridization with a 32P-labeled probe is not optimally suited for routine diagnostic use, this report establishes the feasibility of using nucleic acid hybridization to detect HSV in clinical specimens.

摘要

已开发出一种检测临床标本中单纯疱疹病毒（HSV）DNA的检测方法。它利用核酸杂交技术，使用从克隆在质粒载体中的HSV DNA片段制备的32P标记的DNA探针。该检测方法可以检测到5×10（4）个无细胞HSV的空斑形成单位，以及少至四个病毒感染细胞。与病毒培养法相比，该检测方法在检测生殖器病变拭子标本中的HSV时，灵敏度为78%，特异性为100%。未观察到与未感染、水痘-带状疱疹病毒感染或巨细胞病毒感染细胞的杂交，带状疱疹病变标本均为阴性。虽然与32P标记探针的杂交不太适合常规诊断用途，但本报告确立了使用核酸杂交技术检测临床标本中HSV的可行性。