Shinohara K, Nishikawa T, Ishii S, Yamazaki K, Takahashi K
Division of Mental Disorder Research, National Institute of Neuroscience, Tokyo, Japan.
Neurosci Lett. 1989 Dec 15;107(1-3):307-12. doi: 10.1016/0304-3940(89)90836-7.
The development of N-(1-[2-thienyl]-cyclohexyl)[3H]piperidine [( 3H]TCP) binding to phencyclidine (PCP) receptors in both brain homogenates and slices has been investigated in the rat. The specific binding sites for [3H]TCP in the homogenate were already detected at prenatal stages and steadily increased after birth. A similar developmental pattern was seen in the autoradiography of the [3H]TCP binding to the brain slice in which the distribution of the binding in the young is more homogeneous than that in adult. There was an increase in the Bmax without changes in the Kd of the [3H]TCP binding and there was no change in inhibition of the binding by TCP, PCP and D-(-)-2-amino-5-phosphonovalerate during postnatal maturation. These findings suggest an increase in the density with no change in the affinity of PCP receptors and the absence of a change in the interaction between the PCP and N-methyl-D-aspartate receptors in the developing rat forebrain.
在大鼠中，对脑匀浆和脑片中N-(1-[2-噻吩基]-环己基)[3H]哌啶（[3H]TCP）与苯环己哌啶（PCP）受体结合的发育情况进行了研究。匀浆中[3H]TCP的特异性结合位点在产前阶段就已被检测到，出生后稳步增加。在[3H]TCP与脑片结合的放射自显影中也观察到了类似的发育模式，其中幼龄大鼠结合的分布比成年大鼠更均匀。[3H]TCP结合的Bmax增加，而Kd没有变化，并且在出生后成熟过程中，TCP、PCP和D-(-)-2-氨基-5-磷酸戊酸对结合的抑制作用没有变化。这些发现表明，发育中的大鼠前脑中PCP受体的密度增加，亲和力不变，且PCP与N-甲基-D-天冬氨酸受体之间的相互作用没有变化。
