在一项多中心试验中，通过使用选择性培养基检测囊性纤维化患者中拟青霉属和多育镰孢菌的患病率。
Prevalence of Scedosporium species and Lomentospora prolificans in patients with cystic fibrosis in a multicenter trial by use of a selective medium.
作者信息
Sedlacek L, Graf B, Schwarz C, Albert F, Peter S, Würstl B, Wagner S, Klotz M, Becker A, Haase G, Laniado G, Kahl B, Suerbaum S, Seibold M, Tintelnot K
机构信息
Medizinische Hochschule Hannover, Medizinische Mikrobiologie und Krankenhaushygiene, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
Labor Berlin - Charité Vivantes GmbH, Mikrobiologie/Mykologie, Augustenburger Platz 1, 13353 Berlin, Germany.
出版信息
J Cyst Fibros. 2015 Mar;14(2):237-41. doi: 10.1016/j.jcf.2014.12.014. Epub 2015 Jan 13.
OBJECTIVE
Detection of hyphomycetes of the Scedosporium apiospermum complex and Lomentospora prolificans (Sac-Lp) is not yet standardized. Prevalence rates in patients with cystic fibrosis (CF) and the resistance pattern of these pathogens in Germany are unknown.
METHODS
In a one-year prospective study 11 laboratories used a selective medium for isolation of Sac-Lp, examining >11,600 respiratory samples from 2346 patients with CF. Isolates were identified by molecular methods and tested for susceptibility to antifungal drugs.
RESULTS
The prevalence of Sac-Lp in patients with CF in Germany varied from 0.0 to 10.5% (mean: 3.1%) among the clinical centres. The benefit of the selective medium SceSel(+) compared to standard media for fungi was documented for >5000 samples. High antifungal resistance was detected in the S. apiospermum complex, and the multiresistance of L. prolificans was confirmed.
CONCLUSION
Microbiology laboratories should be aware of these resistant species in patients with CF and consider using a selective medium.
目的
对阿氏丝孢霉复合体和多育镰孢霉（Sac-Lp）的丝状真菌检测尚未标准化。在德国，囊性纤维化（CF）患者中这些病原体的流行率以及耐药模式尚不清楚。
方法
在一项为期一年的前瞻性研究中，11个实验室使用一种选择性培养基分离Sac-Lp，检测了来自2346例CF患者的超过11600份呼吸道样本。通过分子方法鉴定分离株，并检测其对抗真菌药物的敏感性。
结果
在德国的临床中心，CF患者中Sac-Lp的流行率在0.0%至10.5%之间（平均：3.1%）。对于超过5000份样本，记录了选择性培养基SceSel(+)相比于真菌标准培养基的优势。在阿氏丝孢霉复合体中检测到高耐药性，并且多育镰孢霉的多重耐药性得到证实。
结论
微生物实验室应了解CF患者中的这些耐药菌种，并考虑使用选择性培养基。
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