Tilzer L, Thomas S, Moreno R F
Department of Pathology and Oncology, Kansas University Medical Center, Kansas City 66103.
Anal Biochem. 1989 Nov 15;183(1):13-5. doi: 10.1016/0003-2697(89)90163-2.
Phenol and chloroform are the standard solvents used for DNA extraction. These solvents aid in the removal of protein and lipid from crude or partially purified cell extracts. Although the procedure is well established, the solvents are noxious, caustic, and unpleasant. We describe in this paper the use of a special blood collection tube to isolate the offensive organic solvents. With the use of silica gel polymer containing tubes, phenol, phenol:chloroform, or chloroform can be separated from the DNA containing aqueous phase in a rapid and safe manner. The method permits higher yields of DNA since the DNA is poured from the tube rather than aspirated with pipet.
苯酚和氯仿是用于DNA提取的标准溶剂。这些溶剂有助于从粗制或部分纯化的细胞提取物中去除蛋白质和脂质。尽管该程序已成熟，但这些溶剂有毒、有腐蚀性且气味难闻。我们在本文中描述了使用一种特殊的采血管来分离这些有害有机溶剂。通过使用含有硅胶聚合物的管子，可以快速、安全地从含DNA的水相中分离出苯酚、苯酚:氯仿或氯仿。该方法可获得更高的DNA产量，因为DNA是从管中倒出而非用移液器吸取。
