Madej J A, Sobiech K A, Klimentowski S
Arch Exp Veterinarmed. 1989 Nov;43(6):923-7.
Several parameters of protein and carbohydrate metabolism were determined in normal and leukemic lymphocytes in vitro in cattle, including arylamidase activity toward beta-naphthylamides of L-amino acids. The homogenate of bovine leukemic lymphocytes, in comparison with the control revealed increase of gamma-glutamyltransferase, activity trypsin inhibitor and papain inhibitor concentration and aldolase activity. On the other hand, proteolytic activity toward casein and histomucoid content decreased. Out of the 7 substrates used in the study, only 2, alanyl-beta-naphthylamide and leucyl-beta-naphthylamide, demonstrated lower activity in the leukemic material. Disorders in carbohydrate and protein metabolism in the observed lymphocytes in vitro in cattle are presented in the paper.
测定了牛正常淋巴细胞和白血病淋巴细胞体外蛋白质和碳水化合物代谢的几个参数，包括对L-氨基酸β-萘酰胺的芳基酰胺酶活性。与对照组相比，牛白血病淋巴细胞匀浆显示γ-谷氨酰转移酶活性、胰蛋白酶抑制剂活性、木瓜蛋白酶抑制剂浓度和醛缩酶活性增加。另一方面，对酪蛋白的蛋白水解活性和组织黏蛋白含量降低。在该研究中使用的7种底物中，只有2种，即丙氨酰-β-萘酰胺和亮氨酰-β-萘酰胺，在白血病材料中表现出较低的活性。本文介绍了在体外观察到的牛淋巴细胞中碳水化合物和蛋白质代谢的紊乱情况。
