Weremowicz Stanislawa
CAMD-Cytogenetics, Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts.
Curr Protoc Hum Genet. 2015 Jan 20;84:8.8.1-8.8.10. doi: 10.1002/0471142905.hg0808s84.
Numerical and structural chromosome abnormalities can be accurately detected in cells from archived tissues using fluorescence in situ hybridization (FISH). This unit describes two common approaches to performing FISH in formalin-fixed, paraffin-embedded tissue. The first approach utilizes 4 to 6 μm tissue sections in cases for which preserving tissue morphology is necessary, and the second involves extraction of intact nuclei from 50-μm tissue sections. To interpret FISH results using 4 to 6 μm sections, an adequate number of nuclei must be evaluated to perform statistical analysis. Evaluation of 30 to 50 nuclei from the single-cell suspension generally gives an interpretable result.
使用荧光原位杂交（FISH）技术可以在存档组织的细胞中准确检测染色体数目和结构异常。本单元介绍了在福尔马林固定、石蜡包埋组织中进行FISH的两种常见方法。第一种方法适用于需要保留组织形态的情况，使用4至6μm的组织切片；第二种方法是从50μm的组织切片中提取完整的细胞核。要使用4至6μm的切片解释FISH结果，必须评估足够数量的细胞核以进行统计分析。从单细胞悬液中评估30至50个细胞核通常会得到可解释的结果。
