Wang Feng-Qing, Lin Xue-Zheng, Chen Guan-Jun, Du Zong-Jun
College of Marine Science, Shandong University at Weihai, Weihai, 264209, China.
Antonie Van Leeuwenhoek. 2015 Mar;107(3):723-9. doi: 10.1007/s10482-014-0366-2. Epub 2015 Jan 20.
Strain 435(T), a catalase- and oxidase-positive, beige-pigmented, facultatively anaerobic and Gram-stain-negative marine bacterium, was isolated from marine sediment collected in the Arctic (8°21'629''E 72°8'827''N). The cells of the type strain are short- to curve-rods and able to grow at 4-25 °C, pH 6.0-9.0 and in the presence of 0.5-6.0 % (w/v) NaCl. It can reduce nitrate to nitrite. The predominant isoprenoid quinone was identified as Q-8 and the polar lipids are comprised of phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content is 38.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 435(T) belongs to the genus Colwellia. Strain 435(T) was found to exhibit 92.1-95.6 % 16S rRNA gene sequence similarities with other species of the genus Colwellia. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain 435(T) can be considered to represent a novel species, Colwellia arctica sp. nov., of the genus Colwellia. The type strain is 435(T) (=CICC 10860(T) = ATCC BAA-2609(T)).
菌株435(T)是一种过氧化氢酶和氧化酶阳性、呈米色色素、兼性厌氧且革兰氏染色阴性的海洋细菌，从北极采集的海洋沉积物（东经8°21'629''，北纬72°8'827''）中分离得到。模式菌株的细胞为短杆状至弯杆状，能够在4-25°C、pH值6.0-9.0以及0.5-6.0%（w/v）NaCl存在的条件下生长。它能将硝酸盐还原为亚硝酸盐。主要的类异戊二烯醌被鉴定为Q-8，极性脂质由磷脂酰乙醇胺和磷脂酰甘油组成。DNA的G+C含量为38.7 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株435(T)属于科尔韦氏菌属。发现菌株435(T)与科尔韦氏菌属的其他物种在16S rRNA基因序列上具有92.1-95.6%的相似性。其不同的表型特征，以及系统发育和遗传独特性表明，菌株435(T)可被视为代表科尔韦氏菌属的一个新物种，即北极科尔韦氏菌（Colwellia arctica sp. nov.）。模式菌株为435(T)（=CICC 10860(T)=ATCC BAA-2609(T)）。
