Park Sooyeon, Park Ji-Min, Jung Yong-Taek, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-Gu, Suwon, South Korea.
Antonie Van Leeuwenhoek. 2014 Nov;106(5):1041-7. doi: 10.1007/s10482-014-0273-6. Epub 2014 Sep 4.
A Gram-stain negative, aerobic, non-spore-forming, motile and rod-shaped or ovoid bacterial strain, designated GJSW-6(T), was isolated from a seawater collected at Geoje island on the South Sea, South Korea, and subjected to polyphasic taxonomic study. Strain GJSW-6(T) was found to grow optimally at 30 °C, at pH 7.0-8.0 and in the presence of approximately 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GJSW-6(T) clustered with the type strains of two Agarivorans species, Agarivorans albus and Agarivorans gilvus, with which it exhibited sequence similarity values of 99.17 and 95.88 %, respectively. Sequence similarities to the type strains of the other recognized species were less than 93.04 %. Strain GJSW-6(T) was found to contain Q-8 as the predominant ubiquinone and C16:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and C18:1 ω7c as the major fatty acids. The polar lipid profile of strain GJSW-6(T) containing phosphatidylethanolamine and phosphatidylglycerol as major components was similar to those of the type strains of A. albus and A. gilvus. The DNA G+C content of strain GJSW-6(T) was determined to be 45.3 mol% and its mean DNA-DNA relatedness value with A. albus JCM 21469(T) was 19 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GJSW-6(T) is separated from two Agarivorans species. On the basis of the data presented, strain GJSW-6(T) is considered to represent a novel species of the genus Agarivorans, for which the name Agarivorans litoreus sp. nov. is proposed. The type strain is GJSW-6(T) (= KCTC 42116(T) = NBRC 110444(T)). An emended description of the genus Agarivorans is also proposed.
从韩国南海巨济岛采集的海水中分离出一株革兰氏染色阴性、需氧、无芽孢形成、具运动性的杆状或卵形细菌菌株，命名为GJSW-6(T)，并对其进行了多相分类研究。发现菌株GJSW-6(T)在30℃、pH 7.0 - 8.0以及约2.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的系统发育树显示，菌株GJSW-6(T)与两个琼胶ivorans属物种即白琼胶ivorans和吉尔琼胶ivorans的模式菌株聚类，与它们的序列相似性值分别为99.17%和95.88%。与其他已确认物种的模式菌株的序列相似性低于93.04%。发现菌株GJSW-6(T)以Q-8作为主要泛醌，以C16:0、总和特征3（C16:1 ω7c和/或C16:1 ω6c）以及C18:1 ω7c作为主要脂肪酸。菌株GJSW-6(T)的极性脂质谱以磷脂酰乙醇胺和磷脂酰甘油为主要成分，与白琼胶ivorans和吉尔琼胶ivorans的模式菌株相似。菌株GJSW-6(T)的DNA G+C含量测定为45.3 mol%，其与白琼胶ivorans JCM 21,469(T)的平均DNA-DNA相关性值为19%。不同的表型特征，连同系统发育和遗传独特性，表明菌株GJSW-6(T)与两个琼胶ivorans属物种不同。根据所提供的数据，菌株GJSW-6(T)被认为代表琼胶ivorans属的一个新物种，为此提议命名为滨海琼胶ivorans（Agarivorans litoreus）新种。模式菌株为GJSW-6(T)（= KCTC 42,116(T) = NBRC 110,444(T)）。还提议了琼胶ivorans属的修订描述。
