Biocolloid and Fluid Physics Group, Department of Applied Physics, University of Granada, 18071 Granada, Spain.
Group of Material Engineering GEMAT-IQS, University of Ramon Llull, 08017 Barcelona, Spain.
Colloids Surf B Biointerfaces. 2015 Feb 1;126:374-80. doi: 10.1016/j.colsurfb.2014.11.048. Epub 2014 Dec 10.
Two degradable poly(β-amino ester)s with an average molecular weight of 2kDa, referred to as B1 and B2, have been synthesized to be tested as non-viral gene delivery systems. B2 polymer exhibits two additional non-polar ethyl groups at both ends. This paper describes the influence of that subtle difference on the compaction ability and temporal stability of the complexes formed with plasmid DNA. Our results suggest that the inclusion of those small hydrophobic fragments into the polycation backbone improves its suitability as synthetic DNA carrier. The improvement is related to the formation and physicochemical properties of the complexes. B2 polyplexes were more stable, the polymer hydrolysis was slowed down and plasmid DNA was better protected which was translated into better transfection efficiencies. Although still not totally understood, the role played by hydrophobic forces is ubiquitous in chemical, biological and physical systems, and they must be considered to design future polymers for gene delivery.
已经合成了两种平均分子量为 2kDa 的可降解聚（β-氨基酯），分别称为 B1 和 B2，将它们作为非病毒基因传递系统进行测试。B2 聚合物在两端都有两个额外的非极性乙基基团。本文描述了这种微小差异对与质粒 DNA 形成的复合物的压缩能力和时间稳定性的影响。我们的结果表明，将这些小疏水分子片段包含在聚阳离子主链中，可以提高其作为合成 DNA 载体的适用性。这种改进与复合物的形成和物理化学性质有关。B2 聚合物形成的复合物更稳定，聚合物的水解速度减慢，质粒 DNA 得到更好的保护，这转化为更好的转染效率。尽管还不完全清楚，但疏水力在化学、生物和物理系统中无处不在，在设计用于基因传递的未来聚合物时必须考虑到它们。
