Department of Burns & Wound Care Center, Second Affiliated Hospital of Medical College, Zhejiang University, Hangzhou, 310009, China.
J Transl Med. 2018 Feb 15;16(1):29. doi: 10.1186/s12967-018-1402-1.
Critical tissue defects frequently result from trauma, burns, chronic wounds and/or surgery. The ideal treatment for such tissue loss is autografting, but donor sites are often limited. Tissue engineering (TE) is an inspiring alternative for tissue repair and regeneration (TRR). One of the current state-of-the-art methods for TRR is gene therapy. Non-viral gene delivery systems (nVGDS) have great potential for TE and have several advantages over viral delivery including lower immunogenicity and toxicity, better cell specificity, better modifiability, and higher productivity. However, there is no ideal nVGDS for TRR, hence, there is widespread research to improve their properties. This review introduces the basic principles and key aspects of commonly-used nVGDSs. We focus on recent advances in their applications, current challenges, and future directions.
严重的组织缺损通常由创伤、烧伤、慢性创面和/或手术引起。对于此类组织缺失，理想的治疗方法是自体移植，但供体部位往往有限。组织工程（TE）是组织修复和再生（TRR）的一种有前途的替代方法。TRR 的当前最先进方法之一是基因治疗。非病毒基因传递系统（nVGDS）在 TE 中具有巨大的潜力，并且与病毒传递相比具有几个优势，包括更低的免疫原性和毒性、更好的细胞特异性、更好的可修饰性和更高的生产力。然而，目前尚无理想的 TRR 用 nVGDS，因此，广泛开展了改进其性能的研究。本文介绍了常用 nVGDS 的基本原理和关键方面。我们重点介绍了它们在应用中的最新进展、当前挑战和未来方向。
