Ogbebor Nicholas Obehi, Adekunle Adefunke Temitayo, Eghafona Odeh Nosakhare, Ogboghodo Abraham Ikponmwosa
Plant Protection Division, Rubber Research Institute of Nigeria, PMB 1049, Iyanomo, Benin City 300241, Nigeria.
Department of Crop Science, Faculty of Agriculture, University of Benin, PMB 1154, Benin City 300241, Nigeria.
Fungal Biol. 2015 Jan;119(1):1-6. doi: 10.1016/j.funbio.2014.10.002. Epub 2014 Nov 29.
The study was conducted to evaluate in vitro and in vivo control of fungal antagonists on Rigidoporus lignosus (klotzsch) Imaz in Hevea brasiliensis (Willd. ex A. Juss.) Muell. Arg. in the rain forest zone of Nigeria. In vitro assessments of biological agents were carried out in dixenic cultures in Petri plates. In vivo tests were carried out in the nursery using Hypocrea virens and Hypocrea jecorina. Significant differences were observed with the dual inoculations of fungal antagonists and R. lignosus inoculated the same day, and the antagonists inoculated 24 h before inoculation of R. lignosus. Hypocrea jecorina was most effective in the control of R. lignosus with percentage inhibition of 86.83 %. Hypocrea virens, H. jecorina, Trichoderma spirale, Trichoderma sp., Trichoderma sp. Pers, and Hypocrea lixii were effective on R. lignosus. Fungal antagonists inoculated 24 h before inoculation of R. lignosus exhibited higher inhibitory efficacies than antagonists and R. lignosus inoculated the same day. In the in vivo evaluation of fungi antagonists on R. lignosus, seedlings in H. jecorina treatment at 60 d and H. virens treatment at 150 d after inoculations respectively had the highest plant heaths (highest length of stem and length of tap rot, lowest plant death, and least foliar symptom) compared to the control. Mortality rate was higher at 60 d after inoculation and decline from the third months onward. Significant differences were observed between the control and R. lignosus treatment in all the parameters evaluated.
本研究旨在评估在尼日利亚雨林地区，真菌拮抗菌对巴西橡胶树（Willd. ex A. Juss.）Muell. Arg. 上的木蹄层孔菌（klotzsch）Imaz进行体外和体内控制的效果。在培养皿中的双菌培养物中对生物制剂进行体外评估。在苗圃中使用绿粘帚霉和里氏木霉进行体内试验。当真菌拮抗菌与木蹄层孔菌同一天接种，以及拮抗菌在接种木蹄层孔菌前24小时接种时，观察到了显著差异。里氏木霉在控制木蹄层孔菌方面最有效，抑制率为86.83%。绿粘帚霉、里氏木霉、螺旋木霉、木霉属、持久木霉属和利氏木霉对木蹄层孔菌均有效果。在接种木蹄层孔菌前24小时接种的真菌拮抗菌比同一天接种拮抗菌和木蹄层孔菌表现出更高的抑制效果。在对木蹄层孔菌的真菌拮抗菌进行体内评估时，与对照相比，接种后60天里氏木霉处理的幼苗和150天绿粘帚霉处理的幼苗分别具有最高的植株健康状况（最高的茎长和主根长度、最低的植株死亡率和最少的叶片症状）。接种后60天死亡率较高，从第三个月起下降。在所有评估参数中，对照和木蹄层孔菌处理之间均观察到显著差异。
