Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Food Science, College of Agriculture, Islamic Azad University, Yazd, Iran.
Colloids Surf B Biointerfaces. 2015 Feb 1;126:437-43. doi: 10.1016/j.colsurfb.2014.12.047. Epub 2015 Jan 3.
Fumonisin B1 is an important mycotoxin, mainly produced by Fusarium verticillioides. It has toxic effects on liver, brain, and kidney cells. The first aim of this study was to synthesize nanocellulose modified with poly-lysine (NMPL), and the second aim was to evaluate the adsorption of fumonisin B1 by NMPL. As third aim, the function of mouse liver cells was investigated after exposure to fumonisin B1, and fumonisin B1+ NMPL. In this study, NMPL was prepared using cross-linker, and then incubated with fumonisin B1 at controlled conditions. After incubation, the adsorption and release of fumonisin B1 were evaluated in each condition. Next, mouse liver cells were separately exposed to fumonisin B1, NMPL, and (fumonisin B1+NMPL). Then, the level of aniline aminotransferase (ALT) and aspartate aminotransferase (AST) was evaluated. It was found that both adsorption and release of fumonisin B1 were not affected by temperature and incubation time, but affected by pH and concentration of NMPL. Also, this study showed NMPL could adsorb fumonisin B1 in different foodstuffs. Importantly, although the levels of ALT and AST were increased when the cells were treated with fumonisin B1 alone, they were not affected when exposed to NMPL or (fumonisin B1+NMPL). The authors suggest that NMPL is a good adsorbent to remove and inhibit fumonisin B1.
伏马菌素 B1 是一种重要的真菌毒素，主要由轮枝镰孢菌产生。它对肝、脑和肾细胞有毒性作用。本研究的首要目的是合成聚赖氨酸修饰的纳米纤维素（NMPL），其次是评估 NMPL 对伏马菌素 B1 的吸附作用。第三，研究了暴露于伏马菌素 B1 和伏马菌素 B1+NMPL 后小鼠肝细胞的功能。在本研究中，使用交联剂制备 NMPL，然后在控制条件下与伏马菌素 B1 孵育。孵育后，在每种条件下评估伏马菌素 B1 的吸附和释放。接下来，分别将小鼠肝细胞暴露于伏马菌素 B1、NMPL 和（伏马菌素 B1+NMPL）。然后，评估丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）的水平。结果发现，伏马菌素 B1 的吸附和释放既不受温度和孵育时间的影响，也不受 NMPL 的 pH 值和浓度的影响。此外，本研究表明 NMPL 可以吸附不同食品中的伏马菌素 B1。重要的是，尽管当细胞单独用伏马菌素 B1 处理时，ALT 和 AST 的水平升高，但当用 NMPL 或（伏马菌素 B1+NMPL）处理时，它们不受影响。作者认为 NMPL 是一种很好的吸附剂，可以去除和抑制伏马菌素 B1。
