Yildirim Nalan, Agar Guleray
Department of Biology, Faculty of Arts and Sciences, Erzincan University, Erzurum, Turkey
Department of Biology, Faculty of Arts and Sciences, Atatürk University, Erzincan, Turkey.
Toxicol Ind Health. 2016 Aug;32(8):1450-1455. doi: 10.1177/0748233714564416. Epub 2015 Jan 19.
This study was undertaken to appreciate genotoxic potential of fipronil herbicides in the roots of Vicia faba seedlings. Fipronil was clearly dose dependent on root growth inhibitors, total soluble protein, and random amplified polymorphic DNA (RAPD) assays. Increase in concentration of fipronil resulted in decreased amount of root length and increased level of protein. For the RAPD analyses, 15 RAPD primers were found to produce unique polymorphic band patterns and were subsequently used to produce a total of 110 bands of 45-1250 bp. Each primer generated 4-11 RAPD bands across 15 primers. The changes occurring in RAPD profiles following fipronil treatment included variation becoming evident as disappearance and/or appearance of DNA bands compared with the normal seedlings. These results indicated that genomic template stability was significantly affected at the above fipronil concentration. This study further confirmed that the RAPD assays are useful in determining potential genotoxicity of fipronil.
本研究旨在评估氟虫腈除草剂对蚕豆幼苗根系的遗传毒性潜力。氟虫腈在根系生长抑制剂、总可溶性蛋白和随机扩增多态性DNA（RAPD）分析中明显呈剂量依赖性。氟虫腈浓度的增加导致根长度减少和蛋白质水平升高。对于RAPD分析，发现15个RAPD引物产生独特的多态性条带模式，随后用于产生总共110条45 - 1250 bp的条带。每个引物在15个引物中产生4 - 11个RAPD条带。氟虫腈处理后RAPD图谱中出现的变化包括与正常幼苗相比，DNA条带的消失和/或出现使变异变得明显。这些结果表明，在上述氟虫腈浓度下，基因组模板稳定性受到显著影响。本研究进一步证实，RAPD分析可用于确定氟虫腈的潜在遗传毒性。
