Erturk Filiz Aygun, Nardemir Gokce, Hilal A Y, Arslan Esra, Agar Guleray
Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.
Department of Biology, Faculty of Science and Arts, University of Ondokuz Mayis, Samsun, Turkey.
Toxicol Ind Health. 2015 Nov;31(11):1015-23. doi: 10.1177/0748233713485888. Epub 2013 Apr 19.
In this research, we aimed to determine genotoxic effects of boron (B) and zinc (Zn) on Zea mays by using total soluble protein content and random amplification of polymorphic DNA (RAPD) analyses. For the RAPD analysis, 16 RAPD primers were found to produce unique polymorphic band profiles on treated maize seedlings. With increased Zn and B concentrations, increased polymorphism rate was observed, while genomic template stability and total soluble protein content decreased. The treatment with Zn was more effective than that of B groups on the levels of total proteins. The obtained results from this study revealed that the total soluble protein levels and RAPD profiles were performed as endpoints of genotoxicity and these analyses can offer useful biomarker assays for the evaluation of genotoxic effects on Zn and B polluted plants.
在本研究中，我们旨在通过使用总可溶性蛋白含量和随机扩增多态性DNA（RAPD）分析来确定硼（B）和锌（Zn）对玉米的遗传毒性效应。对于RAPD分析，发现16个RAPD引物在处理过的玉米幼苗上产生独特的多态性条带图谱。随着锌和硼浓度的增加，观察到多态性率增加，而基因组模板稳定性和总可溶性蛋白含量降低。在总蛋白水平上，锌处理组比硼处理组更有效。本研究获得的结果表明，总可溶性蛋白水平和RAPD图谱可作为遗传毒性的终点指标，这些分析可为评估锌和硼污染植物的遗传毒性效应提供有用的生物标志物检测方法。
