一种用于扩增小鼠CD8 + T淋巴细胞的可重复方法。
A reproducible method for the expansion of mouse CD8+ T lymphocytes.
作者信息
Lewis Mark D, de Leenheer Evy, Fishman Sigal, Siew Lai Khai, Gross Gideon, Wong F Susan
机构信息
Institute of Molecular and Experimental Medicine, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK.
Laboratory of Immunology, MIGAL Galilee Research Institute, Kiryat Shmona 11016, Israel; Department of Biotechnology, Tel-Hai College, Upper Galilee 12210, Israel.
出版信息
J Immunol Methods. 2015 Feb;417:134-138. doi: 10.1016/j.jim.2015.01.004. Epub 2015 Jan 17.
Murine adoptive CD8+ T-cell immunotherapy studies require the generation of large numbers of high viability CD8+ cells. Here we report a tissue culture protocol for the reliable expansion of CD8+ T-cells derived from murine spleen to give a 20-fold expansion after 4 days in culture. The cells were transfected with an mRNA GFP construct and transferred into NOD mice. GFP positive cells could be detected 7 days after transfer thus confirming that the cells survive and are functional for up to 1 week.
小鼠过继性CD8 + T细胞免疫疗法研究需要大量高活力的CD8 +细胞。在此，我们报告一种组织培养方案，用于可靠地扩增源自小鼠脾脏的CD8 + T细胞，在培养4天后可实现20倍的扩增。将细胞用mRNA绿色荧光蛋白（GFP）构建体转染，然后转移到非肥胖糖尿病（NOD）小鼠体内。转移7天后可检测到GFP阳性细胞，从而证实这些细胞能够存活并在长达1周的时间内保持功能。
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