Shen Qing, Jiang Tao, Li Nan, Wang Jiahui, Han Chunhui, Zhang Jing, Xu Jin, Zhang Dongfeng, Li Fengqin
Medical College, Qingdao University, Qingdao 266021, Shandong Province.
Wei Sheng Yan Jiu. 2014 Nov;43(6):944-52.
The roasted fish fillet sample at the retail collected in Beijing and Qingdao were detected for TTX, and the TTX positive samples was analyzed for fish species identification.
TTX was tested by EUSA method and the cytochrome c oxidase I (COI) genome of TTX-positive samples was extracted and identified by DNA barcode.
Totally, 90 samples were tested by EUSA and 58 (64.4%) samples were positive for TTX with the levels ranging from 0.10 mg/kg to 63.81 mg/kg. Among the TTX positive samples, 24 (41.3%) were identified containing toxic puffer fish and 21 (87.5%) were Lagocephalus lunaris, the highly toxic puffer fish.
Some roasted fish fillet samples obtained from the retail in two cities were positive for TTX and contained toxic puffer fish. Based on these results, we suggest that roasted fish fillet producers should prevent toxic puffer fish from mixing in the raw material and the I regulators should strengthen the TTX surveillance and product labeling supervision of roasted fish fillet.
对在北京和青岛零售渠道采集的烤鱼片样本进行河豚毒素（TTX）检测，并对TTX阳性样本进行鱼类品种鉴定。
采用酶联免疫吸附测定（EUSA）法检测TTX，提取TTX阳性样本的细胞色素c氧化酶I（COI）基因组并通过DNA条形码进行鉴定。
共采用EUSA法检测了90个样本，其中58个（64.4%）样本TTX呈阳性，含量范围为0.10毫克/千克至63.81毫克/千克。在TTX阳性样本中，24个（41.3%）被鉴定含有有毒河豚，其中21个（87.5%）为剧毒河豚月腹刺鲀。
从两个城市零售渠道获得的一些烤鱼片样本TTX呈阳性且含有有毒河豚。基于这些结果，我们建议烤鱼片生产商应防止有毒河豚混入原材料，监管部门应加强对烤鱼片的TTX监测和产品标签监管。
