Kaur Aalamjeet, Bali Anjana, Singh Nirmal, Jaggi Amteshwar Singh
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, 147002, India.
Naunyn Schmiedebergs Arch Pharmacol. 2015 May;388(5):497-507. doi: 10.1007/s00210-015-1084-7. Epub 2015 Jan 21.
The present study was designed to investigate the antistress effect of furosemide (sodium potassium chloride co-transporter inhibitor) in immobilization and foot-shock stress-induced behavioral alterations in the mice. Acute stress was induced in Swiss albino mice either by applying electric foot shocks of 0.6-mA intensity of 1-s duration with 30-s inter-shock interval for 1 h or immobilizing for 150 min. The acute stress-induced behavioral changes were assessed by using actophotometer, hole board, open-field, and social interaction tests. Biochemically, the corticosterone levels were estimated in the serum as a biomarker of hypothalamus-pituitary-adrenal (HPA) axis. Acute stress resulted in the development of behavioral alterations and elevation of the corticosterone levels. Intraperitoneal administration of furosemide (25 and 50 mg/kg) significantly attenuated immobilization and foot-shock stress-induced behavioral changes along with normalization of the corticosterone levels. It may be concluded that furosemide produces beneficial effects in reestablishing the behavioral and biochemical alterations in immobilization and foot-shock-induced acute stress in mice.
本研究旨在探讨呋塞米（钠钾氯共转运体抑制剂）对小鼠固定和足部电击应激诱导的行为改变的抗应激作用。通过施加强度为0.6 mA、持续时间为1 s、电击间隔为30 s的足部电击1小时或固定150分钟，在瑞士白化小鼠中诱导急性应激。使用活动光度计、洞板试验、旷场试验和社交互动试验评估急性应激诱导的行为变化。生化方面，测定血清中皮质酮水平作为下丘脑-垂体-肾上腺（HPA）轴的生物标志物。急性应激导致行为改变和皮质酮水平升高。腹腔注射呋塞米（25和50 mg/kg）可显著减轻固定和足部电击应激诱导的行为变化，并使皮质酮水平恢复正常。可以得出结论，呋塞米在恢复小鼠固定和足部电击诱导的急性应激中的行为和生化改变方面具有有益作用。
