Gao Xin, Zhang Zenghu, Dai Xiaofeng, Zhang Xiao-Hua
College of Marine Life Sciences, Ocean University of China, Qingdao, PR China.
Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, PR China.
Int J Syst Evol Microbiol. 2015 Apr;65(Pt 4):1155-1159. doi: 10.1099/ijs.0.000071. Epub 2015 Jan 20.
A Gram-stain-negative, strictly aerobic, non-flagellated, non-gliding, oxidase- and catalase-positive and rod-shaped yellow-pigmented bacterium, designated strain SW033(T) was isolated from a surface seawater sample collected from the South Pacific Gyre (GPS position: 26° 29' S 137° 56' W) during the Integrated Ocean Drilling Program, expedition 329. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SW033(T) belonged to the genus Hyunsoonleella and showed the highest 16S rRNA gene sequence similarity with Hyunsoonleella jejuensis CNU004(T) (96.8%). It showed 94.7-95.8% 16S rRNA gene sequence similarity with respect to members of the genera Jejuia , Arenitalea and Algibacter in the family Flavobacteriaceae . Optimal growth occurred in the presence of 2-3% (w/v) NaCl, at pH 8.0 and at 28 °C. The genomic DNA G+C content of strain SW033(T) was 36.1 mol%. The major fatty acids were iso-C(15 : 1) G, iso-C(15 : 0), iso-C(17 : 0) 3-OH, iso-C(15 : 0) 3-OH and summed feature 3 (comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c). The major respiratory quinone was menaquinone-6. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids. On the basis of the polyphasic analyses, strain SW033(T) is considered to represent a member of a novel species in the genus Hyunsoonleella , for which the name Hyunsoonleella pacifica sp. nov. is proposed. The type strain is SW033(T) ( = CGMCC 1.11009(T) = JCM 17860(T)).
从综合大洋钻探计划第329航次在南太平洋环流（全球定位系统位置：南纬26°29′，西经137°56′）采集的表层海水样本中分离出一株革兰氏染色阴性、严格需氧、无鞭毛、不滑动、氧化酶和过氧化氢酶阳性、杆状且产黄色色素的细菌，命名为菌株SW033(T)。基于16S rRNA基因序列的系统发育分析表明，菌株SW033(T)属于Hyunsoonleella属，与济州Hyunsoonleella jejuensis CNU004(T)的16S rRNA基因序列相似度最高（96.8%）。它与黄杆菌科Jejuia属、Arenitalea属和Algibacter属成员的16S rRNA基因序列相似度为94.7 - 95.8%。在2 - 3%（w/v）NaCl、pH 8.0和28°C条件下生长最佳。菌株SW033(T)的基因组DNA G + C含量为36.1 mol%。主要脂肪酸为异-C(15 : 1) G、异-C(15 : 0)、异-C(17 : 0) 3-OH、异-C(15 : 0) 3-OH和总和特征3（包括C(16 : 1)ω7c和/或C(16 : 1)ω6c）。主要呼吸醌为甲基萘醌-6。主要极性脂为磷脂酰乙醇胺、两种未鉴定的氨基脂和四种未鉴定的脂。基于多相分析，菌株SW033(T)被认为代表Hyunsoonleella属中的一个新物种，为此提出太平洋Hyunsoonleella pacifica sp. nov.的名称。模式菌株为SW033(T)（= CGMCC 1.11009(T) = JCM 17860(T)）。
