Marine College, Shandong University, Weihai, 264209, PR China.
State key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, PR China.
Int J Syst Evol Microbiol. 2020 Jan;70(1):240-245. doi: 10.1099/ijsem.0.003746.
A novel Gram-stain-negative, strictly aerobic, rod-shaped, non-motile and yellow-pigmented bacterial strain, designated T58, was isolated from a marine sediment sample collected from the coastal area of Weihai, PR China. Strain T58 was most closely related to SW033 with 97.1 % 16S rRNA gene sequence similarity, followed by KCTC 22242 (96.9 %). Based on 16S rRNA gene sequences, the phylogenetic analysis indicated that strain T58 represented a member of the genus within the family of the phylum . Strain T58 was found to grow optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2.0-3.0 % (w/v) NaCl. The major fatty acids were iso-C, iso-C G, iso-C 3-OH and iso-C 3-OH. The major isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The DNA G+C content of the genomic DNA was 35.0 mol%. On the basis of its phylogenetic, phenotypic and chemotaxonomic characteristics, strain T58 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T58 (=KCTC 72081=MCCC 1H00359).
一株新型革兰氏阴性、严格需氧、杆状、非运动、黄色素产生细菌菌株 T58 从中国威海沿海地区的海洋沉积物样本中分离得到。菌株 T58 与 SW033 的 16S rRNA 基因序列相似性最高，为 97.1%，其次是 KCTC 22242（96.9%）。基于 16S rRNA 基因序列，系统发育分析表明，菌株 T58 代表 属内的一个成员，属于 科的 门。菌株 T58 被发现最适生长温度为 30°C，最适 pH 值为 7.0-7.5，最适 NaCl 浓度为 2.0-3.0%（w/v）。主要脂肪酸为 iso-C、iso-C G、iso-C 3-OH 和 iso-C 3-OH。主要类异戊二烯醌为menaquinone 6（MK-6）。主要极性脂为磷脂酰乙醇胺、两种未鉴定的氨基脂和两种未鉴定的脂类。基因组 DNA 的 G+C 含量为 35.0 mol%。基于其系统发育、表型和化学分类特征，菌株 T58 被认为代表了 属的一个新型物种，建议将其命名为. 模式菌株为 T58（=KCTC 72081=MCCC 1H00359）。
