Moon MyeongJu, Thomas Reju George, Heo Seon-U, Park Myong-Suk, Bae Woo Kyun, Heo Suk Hee, Yim Nam Yeol, Jeong Yong Yeon
Department of Radiology, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju, 501-746, South Korea.
Mol Imaging Biol. 2015 Aug;17(4):497-503. doi: 10.1007/s11307-014-0819-z.
In this study, we synthesized hyaluronic acid-conjugated gadolinium (HA-diethylene triamine pentaacetic acid (DTPA)-Gd) and evaluated as hepatocyte-specific magnetic resonance imaging (MRI) contrast agent for the diagnosis of hepatic metastasis.
We conducted Fourier transform (FT)-IR analysis to determine the conjugation of HA and DTPA and performed cell viability assays using NIH3T3 and FL83B cell lines. We also conducted T1-weighted MRI of HA-DTPA-Gd and gadoxetic acid to compare the paramagnetic properties of both.
HA-DTPA-Gd had a higher efficiency in liver MRI compared with the commercially available liver-specific contrast agent (p < 0.001). HA-DTPA-Gd, which possessed a higher T1 relaxivity, showed excellent capability for the diagnosis of hepatic metastasis through an in vivo MRI study in comparison with gadoxetic acid (p < 0.001).
Based on this study, we believe that HA-DTPA-Gd has promising potential for use as a contrast agent for liver MRI of hepatic metastases.
在本研究中，我们合成了透明质酸共轭钆（HA - 二乙烯三胺五乙酸（DTPA）-Gd），并评估其作为用于诊断肝转移的肝细胞特异性磁共振成像（MRI）造影剂。
我们进行了傅里叶变换（FT）-红外分析以确定HA与DTPA的共轭，并使用NIH3T3和FL83B细胞系进行细胞活力测定。我们还对HA - DTPA - Gd和钆塞酸进行了T1加权MRI，以比较两者的顺磁特性。
与市售的肝脏特异性造影剂相比，HA - DTPA - Gd在肝脏MRI中具有更高的效率（p <0.001）。具有更高T1弛豫率的HA - DTPA - Gd，与钆塞酸相比，通过体内MRI研究显示出诊断肝转移的优异能力（p <0.001）。
基于本研究，我们认为HA - DTPA - Gd作为肝转移瘤肝脏MRI造影剂具有广阔的应用前景。
