Wang Linyuan, Guan Ning, Jin Ying, Lin Xiaoping, Gao Hong
Department of Stomatology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China; Department of Periodontics, School of Stomatology of Liaoning Medical College, Jinzhou, Liaoning, China.
Key Laboratory of Brain and Spinal Cord Injury Research, First Affiliated Hospital of Liaoning Medical College, Jinzhou, Liaoning, China.
Int Immunopharmacol. 2015 Mar;25(1):65-73. doi: 10.1016/j.intimp.2015.01.007. Epub 2015 Jan 18.
To date, Porphyromonas gingivalis (P. gingivalis) vaccination has been studied only in animals, and no effective prophylactic human periodontal vaccine has been developed, with the reason for the failure of prophylactic human periodontal vaccines unknown. T helper 17 cell (Th17)/regulatory T (Treg) cell responses play an important role in the development of periodontitis, and a Th17/Treg imbalance causes the pathogenesis of periodontitis. However, whether vaccination with P. gingivalis can prevent periodontitis through modulation of the Th17/Treg imbalance remains unknown. In this study, mice were subcutaneously vaccinated with formalin-killed P. gingivalis and then orally challenged with P. gingivalis. The vaccination protected the mice from alveolar bone resorption and inflammation. These protective effects might be ascribed to downregulation of Th17 cells and interleukin (IL)-17A production, upregulation of Treg and receptor activator of nuclear factor-kappa B ligand (RANKL)(+)CD4(+)T cells, and IL-10 and transforming growth factor-β1 production, and inhibition of lymphocyte proliferation. Our findings may provide a direction for the development of a vaccine or therapy against periodontitis by alteration of the Th17/Treg imbalance.
迄今为止，牙龈卟啉单胞菌（P. gingivalis）疫苗仅在动物身上进行了研究，尚未开发出有效的预防性人牙周疫苗，预防性人牙周疫苗失败的原因尚不清楚。辅助性T细胞17（Th17）/调节性T（Treg）细胞反应在牙周炎的发生发展中起重要作用，Th17/Treg失衡导致牙周炎的发病机制。然而，牙龈卟啉单胞菌疫苗接种是否能通过调节Th17/Treg失衡来预防牙周炎仍不清楚。在本研究中，用福尔马林灭活的牙龈卟啉单胞菌对小鼠进行皮下接种，然后用牙龈卟啉单胞菌进行口服攻击。接种疫苗可保护小鼠免受牙槽骨吸收和炎症的影响。这些保护作用可能归因于Th17细胞和白细胞介素（IL）-17A产生的下调、Treg和核因子κB受体激活剂配体（RANKL）（+）CD4（+）T细胞的上调、IL-10和转化生长因子-β1的产生以及淋巴细胞增殖的抑制。我们的研究结果可能为通过改变Th17/Treg失衡来开发抗牙周炎疫苗或疗法提供一个方向。
